Biological control of maize sheath blight caused by Rhizoctonia solani using rhizosphere fungi isolated from wild Solanum lasiocarpum Dunal: an in vitro study
Abstract
Maize sheath blight caused by Rhizoctonia solani is a major constraint to maize production, particularly in tropical peatland ecosystems where environmental conditions favor pathogen development. This study evaluated the antagonistic potential of rhizosphere fungi isolated from the indigenous plant Solanum lasiocarpum Dunal (Terung Asam) against R. solani under in vitro conditions. Rhizosphere fungi were isolated and identified using morphological characteristics and ITS rDNA sequencing, while their antagonistic activity and volatile organic compound (VOC)-mediated inhibition were assessed using dual culture and sealed plate assays. Six fungal isolates were successfully recovered from the rhizosphere of S. lasiocarpum and identified as belonging to the genera Trichoderma (TA-1–TA-3) and Aspergillus (TA-4–TA-6). Among them, Trichoderma isolate TA-1 (82.8%) and Aspergillus isolate TA-4 (81.4%) exhibited the highest inhibition of R. solani, indicating strong antagonistic activity through competition, mycoparasitism, and antibiosis. In the VOC assay, Aspergillus isolates TA-4 and TA-5 showed the strongest inhibition of pathogen growth, suggesting the production of antifungal volatile metabolites. Molecular identification based on ITS sequencing and BLAST analysis confirmed that isolate TA-1 was Trichoderma asperellum (576 bp) and isolate TA-4 was Aspergillus welwitschiae (574 bp), both sharing 100% sequence similarity with reference sequences in GenBank. To our knowledge, this is the first report describing antagonistic fungi isolated from the rhizosphere of wild S. lasiocarpum as potential biological control agents against maize sheath blight caused by R. solani. These indigenous fungi represent promising candidates for the development of environmentally sustainable disease management strategies in tropical peatland agriculture.
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Adhi SR & Suganda T. 2020. Potensi jamur rizosfer bawang merah menekan Fusarium oxysporum f.sp. cepae, penyebab penyakit busuk umbi bawang merah [The potential of shallot rhizospheric fungi in suppressing Fusarium oxysporum f.sp. cepae, the causal agent of basal rot disease]. Jurnal Kultivasi. 19(1): 1015–1022. https://doi.org/10.24198/kultivasi.v19i1.22877
Agustina D, Triasih U, Dwiastuti ME, & Wicaksono RC. 2019. Potensi jamur antagonis dalam menghambat pertumbuhan jamur Botryodiplodia theobromae penyebab penyakit busuk batang pada tanaman jeruk [Potential of antagonistic fungi in inhibiting the growth of Botryodiplodia theobromae fungi causes stem rot disease in citrus]. Jurnal Agronida. 5(1): 1–6.
Aizah SM, Purnawati A, & Prasetyawati ET. 2024. Potential of Trichoderma sp. the origin of peat soil as a biological control agent for pathogenic fungi carried by rice seeds. BIOEDUSCIENCE. 8(3): 352–358. https://doi.org/10.22236/jbes/15354
Amaria W, Harni R, & Samsudin. 2015. Evaluasi jamur antagonis dalam menghambat pertumbuhan Rigidoporus microporus penyebab penyakit jamur akar putih pada tanaman karet [Evaluation of antagonistic fungi in inhibiting the growth of Rigidoporus microporus causing white root disease in rubber plants]. J. TIDP. 2(1): 51–60.
Anggraeni DN & Usman M. 2015. Uji aktivitas dan identifikasi jamur rhizosfer pada tanah perakaran tanaman pisang (Musa paradisiaca) terhadap jamur fusarium [Test activity and identification of fungi on soil crop root rhizosphere banana (Musa paradisiaca) against fusarium]. BioLink. 1(2): 89–98. https://doi.org/10.31289/biolink.v1i2.729
Ariyanti AEL, Suriani, & Wahab SS. 2021. Potensi mikroba antagonis Bacillus cereus dan Trichoderma sp. terhadap patogen penting tanaman jagung [Anthagonic microbial potential of Bacillus cereus and Trichoderma sp. on the important pathogen of corn plant]. Tarjih Agriculture System Journal. 1(1): 23–29.
Armila Z, Ambar AA, Ilmi N, Harsani H, & Rahim I. 2019. Potensi jamur Trichoderma sp. pengendalian Phytophthora palmivora secara in vitro [Potential of Trichoderma sp. fungus in control of Phytophthora palmivora in vitro]. Prosiding Seminar Nasional 2019 Sinergitas Multidisiplin Ilmu Pengetahuan dan Teknologi. 2: 255–258.
Barnett HL & Hunter BB. 1998. Illustrated Genera of Imperfect Fungi. 4th edition. Burgess Publishing Company. United States of America.
Bernadip BR, Hadiwiyono, & Sudadi. 2014. Keanekaragaman jamur dan bakteri rizosfer bawang merah terhadap patogen moler [Diversity of fungi and bacteria of shallot rizosphere against moler pathogen]. Sains Tanah: Jurnal Ilmu Tanah dan Agroklimatologi. 11(1): 52–60.
de Carvalho Cardoso ES, Martins NQ, Azevedo RA, Palmeira LS, Quintanilha-Peixoto G, Andrade B, Santos MPF, Uetanabaro APT, da Silva EGP, Góes-Neto A, & da Costa AM. 2025. Production and application of inulinase by new isolates of Aspergillus welwitschiae from fermented peach-palm waste for the production of fructooligosaccharides. Food Chem. 465(1): 141978. https://doi.org/10.1016/j.foodchem.2024.141978
Di R, Liu L, Shoaib N, Xi B, Zhou Q, & Yu G. 2023. Sheath blight of maize: An overview and prospects for future research directions. Agriculture. 13(10): 2006. https://doi.org/10.3390/agriculture13102006
Duarte EAA, Damasceno CL, de Oliveira TAS, Barbosa LdO, Martins FM, de Queiroz Silva JR, de Lima TEF, da Silva RM, Kato RB, Bortolini DE, Azevedo V, Góes-Neto A, & Soares ACF. 2018. Putting the mess in order: Aspergillus welwitschiae (and not A. niger) is the etiological agent of sisal bole rot disease in Brazil. Front. Microbiol. 9: 1227. https://doi.org/10.3389/fmicb.2018.01227
El Jaddaoui I, Rangel DEN, & Bennett JW. 2023. Fungal volatiles have physiological properties. Fungal Biol. 127(7–8): 1231–1240. https://doi.org/10.1016/j.funbio.2023.03.005
Fahrul ZACP. 2024. Pemetaan produksi tanaman jagung di Provinsi Jawa Tengah dengan berbasis sistem informasi geografis [Mapping maize production in Central Java Province using a geographical information-based approach]. Jurnal Ilmiah Sains Teknologi dan Informasi. 2(1): 30–34. https://doi.org/10.59024/jiti.v2i1.594
Herrera W, Valbuena O, & Pavone-Maniscalco D. 2020. Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings. Egypt. J. Biol. Pest Control. 30(1): 44. https://doi.org/10.1186/s41938-020-00246-9
Husna, Hussain A, Shah M, Hamayun M, Qadir M, & Iqbal A. 2021. Heavy metal tolerant endophytic fungi Aspergillus welwitschiae improves growth, ceasing metal uptake and strengthening antioxidant system in Glycine max L. Environ. Sci. Pollut. Res. 29(11): 15501–15515. https://doi.org/10.1007/s11356-021-16640-1
Ibrahim NF, Zakaria NA, & Aris F. 2022.Phytochemistry and aiological activity of terung asam, indigenous fruit-vegetables of Sarawak - A review. J. Sustain. Sci. Manag. 17(2): 270–285.
Iswati R, Aini LQ, Soemarno, & Abadi AL. 2024. Exploration and characterization of indigenous Trichoderma spp. as antagonist of Rhizoctonia solani and plant growth promoter of maize. Biodiversitas. 25(4): 1375–1385. https://doi.org/10.13057/biodiv/d250405
Jati WW, Kristini A, Mulyatni AS, & Abadi AL. 2023. Identifikasi molekuler Trichoderma sp. isolat T10 ISRI sebagai agen hayati penyakit busuk akar dan pangkal batang Xylaria [Molecular identification of Trichoderma sp. T10 ISRI isolate as a biological agent of root and stem rot disease of Xylaria]. Indonesian Sugar Research Journal. 3(1): 25–32. https://doi.org/10.54256/isrj.v3i1.94
Juliana, Parawansa AK, & Nontji M. 2024. Inventarisasi serangan penyakit busuk pelepah (Rhizoctonia solani Khun) pada tanaman jagung (Zea mays L.) di Kabupaten Maros [Inventory of sheath blight disease (Rhizoctonia solani Khun) attack on corn (Zea Mays L.) in Maros Regency]. Jurnal AGrotekMAS. 5(2): 196–202. https://doi.org/10.33096/agrotekmas.v5i2.610
Kamble MV, Joshi SM, Hadimani S, & Jogaiah S. 2021. Biopriming with rhizosphere Trichoderma harzianum elicit protection against grapevine downy mildew disease by triggering histopathological and biochemical defense responses. Rhizosphere. 19: 100398. https://doi.org/10.1016/j.rhisph.2021.100398
Khairul I, Montong VB, & Ratulangi MM. 2017. Uji antagonisme Trichoderma sp. terhadap Colletotrichum capsici penyebab penyakit antraknosa pada cabai keriting secara in vitro [Test antagonism Trichoderma sp. against Colletotrichum capsica causes anthracnose disease in chili curly in vitro]. Cocos. 9(6): 1–8.
Khuong NQ, Ngoc LY, Thuan VM, Hiep PC, Trong ND, Quang LT, Thu LTM, Huy NG, Nhan TC, Xuan LNT, & Xuan DT. 2025. Isolating, characterizing, and utilizing Trichoderma asperellum to antagonize Neurospora spp. causing scab disease on King Mandarin fruits (Citrus sinensis). J Trop Plant Pests Dis. 25(1): 141–157. https://doi.org/10.23960/jhptt.125141-157
Kubiak A, Wolna-Maruwka A, Pilarska AA, Niewiadomska A, & Piotrowska-Cyplik A. 2023. Fungi of the Trichoderma genus: Future perspectives of benefits in sustainable agriculture. Appl. Sci. 13(11): 6434. https://doi.org/10.3390/app13116434
Kumalasari AS, Mustaka ZD, & Hendra. 2021. Keefektifan daun sirih (Piper betle Linn.) dalam menekan patogen pelepah (Rhizoctonia solani) pada tanaman jagung [The effectiveness of betel leaf (Piper betle Linn.) in suppressing the sheath pathogen (Rhizoctonia solani) in corn plants]. Tarjih Agriculture System Journal. 1(2): 60–66.
Li J, Zhang A, Liu Y, Zhang Y, & Gu R. 2023. Isolation, identification, and host range of Aspergillus welwitschiae causing postharvest rot on Chinese cabbage in China. Can. J. Plant Pathol. 45(4): 351–359. https://doi.org/10.1080/07060661.2023.2208088
Matondang STD & Aini LQ. 2022. Eksplorasi jamur rizosfer antagonis terhadap Rhizoctonia solani pada tanaman kacang hijau (Vigna radiata) [Exploration of antagonist rhizosphere fungi against Rhizoctonia solani on mung bean plants (Vigna radiata)]. Jurnal HPT. 10(2): 85–96 https://doi.org/10.21776/ub.jurnalhpt.2022.010.2.4
Mirsam H, Suriani, Kurniawati S, Purwanto OD, Muis A, Pakki S, Tenrirawe A, Nonci N, Herawati, Muslimin, & Azrai M. 2023. In vitro inhibition mechanism of Trichoderma asperellum isolates from corn against Rhizoctonia solani causing banded leaf and sheath blight disease and its role in improving the growth of corn seedlings. Egypt. J. Biol. Pest Control. 33(1): 95. https://doi.org/10.1186/s41938-023-00729-5
Mukherjee PK, Mendoza-Mendoza A, Zeilinger S, & Horwitz BA. 2022. Mycoparasitism as a mechanism of Trichoderma-mediated suppression of plant diseases. Fungal Biol. Rev. 39: 15–33. https://doi.org/10.1016/j.fbr.2021.11.004
Mulyani RB, Djaya AA, Silitonga IL, Supriati L, Padriyani, & Melhanah. 2024. The role antagonistic fungus of Solanum lasiocarpum rhizophere to control chili seed borne pathogen in vitro. IOP Conf. Ser.: Earth Environ. Sci. 1421: 012013. https://doi.org/10.1088/1755-1315/1421/1/012013
Mukaromah BD, Bahri S, & Indahsari WMN. 2025. Exploration and identification of Gliocladium sp. as biological agent from the rhizosphere of tomato. Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH). 12(1): 1–12. https://doi.org/10.23960/jbekh.v12i1.390
Muslim A. 2019. Pengendalian Hayati Patogen Tanaman dengan Mikroorganisme Antagonis [Biological Control of Plant Pathogens with Antagonistic Microorganisms]. Unsri Press. Palembang.
Myat AC & Myint KO. 2024. Morphological, microscopical and phytochemical studies on leaves of Solanum lasiocarpum Dunal. 4th Myanmar Korea Conference Research Journal. 4(1): 1–8.
Ngo MT, Nguyen MV, Han JW, Kim B, Kim YK, Prak MS, Kim H, & Choi GJ. 2021. Biocontrol potential of Aspergillus species producing antimicrobial metabolites. Front Microbiol. 12: 804333. https://doi.org/10.3389/fmicb.2021.804333
Ningsih IS, Chatri M, Advinda L, & Violita. 2023. Senyawa aktif flavonoid yang terdapat pada tumbuhan [Flavonoid active compounds found in plants]. Serambi Biologi. 8(2): 257–263.
Nion YA & Kamilah. 2023. The potential of organic vegetable from peat awamp land in Central Kalimantan as fungicide and bactericide. J. Wetlands. Environ. Manag. 11(1): 44–55.
Nofal AM, El-Rahman MA, Abdelghany TM, & Abd El-Mongy M. 2021. Mycoparasitic nature of Egyptian Trichoderma isolates and their impact on suppression Fusarium wilt of tomato. Egypt. J. Biol. Pest Control. 31(1): 103. https://doi.org/10.1186/s41938-021-00450-1
Picardal JP, Tundag EDL, Picardal MT, & Goc-ong GB. 2019. Antagonistic activity of Metarhizium anisopliae (Metschnikoff) against phytopathogenic Fusarium oxysporum f. sp. cubense (Schlecht.) as a biological control. CNU J. High. Educ. 13(1): 4. https://doi.org/10.70997/2546-1796.1014
Rahman ZA, Zaidan MWAM, Othman AN, Ahmad MA, Simoh S, & Ismail MAH. 2019. Optimizing extraction of phenolics and flavonoids from Solanum ferox fruit. Natural Science. 11(4): 99–105. https://doi.org/10.4236/ns.2019.114011
Rajani P, Rajasekaran CR, Vasanthakumari MM, Olsson SB, Ravikanth G, & Shaanker U. 2021. Inhibition of plant pathogenic fungi by endophytic Trichoderma spp. through mycoparasitism and volatile organic compounds. Microbiol. Res. 242: 126595. https://doi.org/10.1016/j.micres.2020.126595
Razo-Belmán R, Ángeles-López YI, García-Ortega LF, León-Ramírez CG, Ortiz-Castellanos L, Yu H, & Martínez-Soto D. 2023. Fungal volatile organic compounds: Mechanisms involved in their sensing and dynamic communication with plants. Front. Plant Sci. 14: 1257098. https://doi.org/10.3389/fpls.2023.1257098
Rotasouw SM, Taribuka J, & Amanupunyo HRD. 2020. Identifikasi dan kemampuan jamur endofitik asal jagung (Zea mays L.) terhadap patogen busuk pelepah (Rhizoctonia solani) [Identification and ability of endophytic fungus from corn (Zea mays L.) against midrib rot pathogens (Rhizoctonia solani)]. Jurnal Budidaya Pertanian. 16(2): 140–146. https://doi.org/10.30598/jbdp.2020.16.2.140
Rushdy MNQ, Benjamin MAZ, & Awanga MA. 2025. Optimising terung asam (Solanum lasiocarpum Dunal.) sauce for enhanced phenolics, flavonoids, and antioxidant capacity with physicochemical properties and storage stability analysis. Malays. J. Fundam. Appl. Sci. 21(1): 1697–1708. https://doi.org/10.11113/mjfas.v21n1.4010
Sarkar AK & Sadhukhan S. 2023. Unearthing the alteration in plant volatiles induced by mycorrhizal fungi: A shield against plant pathogens. Physiol. Plant. 175(1): e13845. https://doi.org/10.1111/ppl.13845
Simanjuntak CP & Safni I. 2025. Detection and identification of fungi causing strawberry wilt disease in North Sumatra, Indonesia. J Trop Plant Pests Dis. 25(1): 32–43. https://doi.org/10.23960/jhptt.12532-43
Soenartiningsih, Djaenuddin N, & Saenong MS. 2014. Efektivitas Trichoderma sp. dan Gliocladium sp. sebagai agen biokontrol hayati penyakit busuk pelepah daun pada jagung [Efficacy of Trichoderma sp. and Gliocladium sp. to control sheath blight disease on maize]. Penelitian Pertanian Tanaman Pangan. 33 (2): 129–135.
Soenartiningsih, Akil M, & Andayani NN. 2015. Cendawan tular tanah (Rhizoctonia solani) penyebab penyakit busuk pelepah pada tanaman jagung dan sorgum dengan komponen pengendaliannya [Soil borne fungus (Rhizoctonia solani) the pathogen of sheath blight disease of maize and sorghum and its control measures]. Buletin Iptek Tanaman Pangan. 10(2): 85–91.
Soon ATK & Ding P. 2021. A review on wild indigenous eggplant, terung asam Sarawak (Solanum lasiocarpum Dunal). Sains Malaysiana. 50(3): 595–603 https://doi.org/10.17576/jsm-2021-5003-03
Soplanit RC, Patty J, & Talahaturuson A. 2021. Antagonisme in vitro lima isolat lokal Trichoderma spp. asal rhizosfer terhadap Rhizoctonia solani penyebab busuk pelepah jagung [In vitro antagonisms of five Trichoderma spp. local isolates of rhizosphere origin against Rhizoctonia solani causing of corn sheath rot]. Jurnal Budidaya Pertanian. 17(2): 89–98.
Suciati F, Anwar S, Dadang, Aviantara DB, & Widyastuti R. 2016. Pengaruh pemberian pestisida terhadap transformasi asam fenolat serta produksi CO2 dan CH4 pada tanah gambut [Effects of pesticide application on phenolic acid transformations and CO2 and CH4 productions from peat soil]. Jurnal Tanah dan Iklim. 40(1): 11–22.
Sumartini. 2012. Penyakit tular tanah (Sclerotium rolfsii dan Rhizoctonia solani) pada tanaman kacang kacangan dan umbi-umbian serta cara pengendaliannya [Soil-borne diseases caused by Rhizoctonia solani and Sclerotium rolfsii on legumes and tuber crops and their control]. Jurnal Litbang Pertanian. 31(1): 27–34.
Supriati L, Melhanah, Oemar O, & Milenia S. 2023. Eksplorasi jamur agens hayati dari rhizosfir di lahan gambut dengan pola tanam yang berbeda [Exploration of fungal biological agents from the rhizosphere in peatlands with different planting patterns]. Jurnal AGRI PEAT. 24(2): 32–39. https://doi.org/10.36873/agp.v24i2.8658
Syarifah SM, Sari OP, & Bimantara A. 2024. Pengendalian hayati patogen Fusarium oxysporum f. sp. capsici dengan isolat Trichoderma sp. asal rizosfer bambu dari Kcamatan Kedu, Kabupaten Temanggung [Pathogen biological control of Fusarium oxysporum f. sp. capsici using isolated Trichoderma sp. of bamboo rhizosphere from Kedu District, Temanggung Regency]. Jurnal Ilmu Pertanian Indonesia. 29(3): 454–460. https://doi.org/10.18343/jipi.29.3.454
Watanabe T. 2002. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species. CRC press. Boca Raton.
Ying L, Zhong D, De-liang P, Shi-ming L, Ling-an K, Huan P, Chao X, Zhong-cai L, & Wen-kun H. 2019. Evaluation of the biocontrol potential of Aspergillus welwitschiae against the root-knot nematode Meloidogyne graminicola in rice (Oryza sativa L.). J. Integr. Agric. 18(11): 2561–2570. https://doi.org/10.1016/S2095-3119(19)62610-9
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