Assessment of cassava mosaic disease (CMD) intensity and whitefly (Bemisia tabaci) population in Cameroon based on field surveys
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Abstract
Cassava mosaic disease (CMD) remains a major constraint to cassava (Manihot esculenta Crantz) production in Cameroon. This study aimed to generate updated epidemiological data on CMD across the country. Surveys were conducted in 342 fields across the ten administrative regions of Cameroon between January and September 2022, following a standardized protocol adopted by all fourteen countries participating in the Central and West African Virus Epidemiology for Food Security (WAVE) program. In each field, 30 plants were assessed for CMD incidence, severity, whitefly population, and mode of infection. CMD symptoms were observed in all ten regions. The overall mean CMD incidence was 54.72%, with a mean severity score of 2.39. Incidence, severity, and whitefly populations varied significantly among regions. The South Region recorded the highest mean incidence (74.34%), while the Far North had the lowest (18.97%). The Adamawa Region exhibited the highest mean severity (2.63), whereas the South West Region had the lowest (2.19). Whitefly abundance per plant was greatest in the South West (21.44) and lowest in the Far North (0.40). PCR amplification and sequencing confirmed the presence of cassava mosaic geminiviruses (CMGs) in leaf samples. A positive correlation was detected between mean whitefly abundance and CMD incidence, while altitude was negatively correlated with whitefly numbers. Both CMD incidence and whitefly populations were higher in intercropped fields than in monocropped fields. Notably, intercropping cassava with maize reduced whitefly populations, while intercropping with sweet potato lowered CMD incidence. The primary source of CMD infection was the use of infected cuttings. These findings provide valuable insights for the development of targeted interventions and improved management strategies for CMD in Cameroon.
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References
Abubakar M, Mohammed IU, Anas H, & Mohammed MT. 2019. Severity of cassava mosaic disease in North East, Nigeria. JIRLS. 1(1): 1–8. https://www.jirlsonline.com/?mno=108378
Akinbade SA, Hanna R, Nguenkam A, Njukwe E, Fotso A, Doumtsop A, Ngeve J, Tenku STN, & Kumar PL. 2010. First report of the East African cassava mosaic virus-Uganda (EACMV-UG) infecting cassava (Manihot esculenta) in Cameroon. New. Dis. Rep. 21(1): 22. https://doi.org/10.5197/j.2044-0588.2010.021.022
Alabi OJ, Kumar PL, & Naidu RA. 2008. Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava. J. Virol. Methods. 154(1–2): 111–120. https://doi.org/10.1016/j.jviromet.2008.08.008
Alain H, Bertrand MS, Abdou KNN, Sylvere DLL, Patrice NDJ, & Zachee A. 2024. Prevalence of African mosaic and morphological characterization of cassava (Manihot esculenta Cranz) varieties in two agroecological zones of Cameroon. J. Exp. Agric. Int. 46(6): 222–249. https://doi.org/10.9734/jeai/2024/v46i62474
Ally HM, Hamss HE, Simiand C, Maruthi MN, Colvin J, Omongo CA, & Delatte H. 2019. What has changed in the outbreaking populations of the severe crop pest whitefly species in cassava in two decades? Sci. Rep. 9: 14796. https://doi.org/10.1038/s41598-019-50259-0
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, & Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25(17): 3389–3402. https://doi.org/10.1093/nar/25.17.3389
Aregbesola OZ, Legg JP, Lund OS, Sigsgaard L, Sporleder M, Carhuapoma P, & Rapisarda C. 2020. Life history and temperature-dependence of cassava-colonising populations of Bemisia tabaci. J. Pest Sci. 93: 1225–1241 https://doi.org/10.1007/s10340-020-01249-z
Biola CF, Mukendi RT, Kalonji-Mbuyi A, & Nkongolo KK. 2022. Epidemiological assessment of cassava mosaic disease in a savanna region of the Democratic Republic of Congo. Int. J. Sustain. Agric. Res. 9(4): 168–182. https://doi.org/10.18488/ijsar.v9i4.3220
Caspary R, Wosula EN, Issa KA, Amour M, & Legg JP. 2023. Cutting dipping application of flupyradifurone against cassava whiteflies Bemisia tabaci and impact on its parasitism in cassava. Insects. 14(10): 796. https://doi.org/10.3390/insects14100796
Changadeya W, Kamowa-Mbewe W, Kumar L, Ntawuruhunga P, Legg JP, Fauquet CM, & Mkumbira J. 2016. The prevalence of Cassava mosaic begomoviruses in Malawi. IJRSS. 6(1): 70–87.
Chikoti PC, Mulenga RM, Tembo M, & Sseruwagi P. 2019. Cassava mosaic disease: A review of a threat to cassava production in Zambia. J. Plant Pathol. 101: 467–477. https://doi.org/10.1007/s42161-019-00255-0
Chikoti PC, Tembo M, Legg JP, Shirima RR, Mugerwa H, & Sseruwagi P. 2020. Genetic diversity of mitochondrial DNA of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) associated with cassava and the occurrence of cassava mosaic disease in Zambia. Insects. 11(11): 761. https://doi.org/10.3390/insects11110761
Chikoti PC & Tembo M. 2022. Expansion and impact of cassava brown streak and cassava mosaic diseases in Africa: A review. Front. Sustain. Food Syst. 6: 1076364. https://doi.org/10.3389/fsufs.2022.1076364
Claude KZG, N’zue B, & Brown JK. 2023. A molecular study on African cassava mosaic disease management in Côte d’Ivoire. Int. J. Plant Pathol. 14(1): 1–12. https://doi.org/10.3923/ijpp.2023.1.12
Doungous O, Masky B, Levai DL, Bahoya JAL, Minyaka E, Mavoungou JF, Mutuku JM, & Pita JS. 2022. Cassava mosaic disease and its whitefly vector in Cameroon: Incidence, severity and whitefly numbers from field surveys. Crop Prot. 158: 106017. https://doi.org/10.1016/j.cropro.2022.106017
Dye AE, Muga B, Mwangi J, Hoyer JS, Ly V, Rosado Y, Sharpee W, Mware B, Wambugu M, Labadie P, Deppong D, Jackai L, Jacobson A, Kennedy G, Ateka E, Duffy S, Hanley-Bowdoin L, Carbone I, & Ascencio-Ibáñez JT. 2023. Cassava begomovirus species diversity changes during plant vegetative cycles. Front. Microbiol. 14: 1163566. https://doi.org/10.3389/fmicb.2023.1163566
Eni AO, Efekemo OP, Onile-ere OA, & Pita JS. 2021. South West and North Central Nigeria: Assessment of cassava mosaic disease and field status of African cassava mosaic virus and East African cassava mosaic virus. Ann. Appl. Biol. 178(3): 466–479. https://doi.org/10.1111/aab.12647
Evouna JSM & Ngounou BA. 2024. Analysis of the volatility of the price of cassava in Cameroon: Implications for food security. GSSR. 3(2): 3658. https://doi.org/10.56556/gssr.v3i2.960
Ewusie EA, Parajulee MN, Adabie-Gomez DA, & Wester D. 2010. Strip cropping: A potential IPM tool for reducing whitefly, Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) infestations in cassava. West Afr. J. Appl. Ecol. 17: 109–119.
FAO 2017. Food Outlook: Binnual Report on Global Food Markets. https://reliefweb.int/sites/reliefweb.int/files/resources/a-I8080e.pdf. Accessed 31 January 2024.
FAOSTAT. 2025. Online Statistical Database: Food Balance. http://fao.org/faostat/fr/#data/QCL/visualize. Accessed 4 September 2025.
Fondong VN, Pita JS, Rey MEC, de Kochko A, Beachy RN, & Fauquet CM. 2000. Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon. J. Gen. Virol. 81(Pt 1): 287–297. https://doi.org/10.1099/0022-1317-81-1-287
Fondong VN, Thresh JM, & Zok S. 2002. Spatial and temporal spread of cassava mosaic virus disease in cassava grown alone and when intercropped with maize and/or cowpea. J. Phytopathol. 150(7): 365–374. https://doi.org/10.1046/j.1439-0434.2002.00775.x
Hahn SK, Terry ER, & Leuschner K. 1980. Breeding cassava for resistance to cassava mosaic disease. Euphytica. 29: 673–683. https://doi.org/10.1007/BF00023215
Hareesh PS, Resmi TR, Sheela MN, & Makeshkumar T. 2023. Cassava mosaic disease in South and Southeast Asia: Current status and prospects. Front. Sustain. Food Syst. 7: 1086660. https://doi.org/10.3389/fsufs.2023.1086660
Houngue JA, Pita JS, Cacaï GHT, Zandjanakou-Tachin M, Abidjo EAE, & Ahanhanzo C. 2018. Survey of farmers’ knowledge of cassava mosaic disease and their preferences for cassava cultivars in three agro-ecological zones in Benin. J Ethnobiology Ethnomedicine. 14: 29. https://doi.org/10.1186/s13002-018-0228-5
Houngue JA, Zandjanakou-Tachin M, Ngalle HB, Pita JS, Cacaï GHT, Ngatat SE, Bell JM, & Ahanhanzo C. 2019. Evaluation of resistance to cassava mosaic disease in selected African cassava cultivars using combined molecular and greenhouse grafting tools. Physiol. Mol. Plant Pathol. 105: 47–53. https://doi.org/10.1016/j.pmpp.2018.07.003
Houngue JA, Houédjissin SS, Ahanhanzo C, Pita JS, Houndénoukon EMS, & Zandjanakou-Tachin M. 2022. Cassava mosaic disease (CMD) in Benin: Incidence, severity and its whitefly abundance from field surveys in 2020. Crop Prot. 158: 106007. https://doi.org/10.1016/j.cropro.2022.106007
INS 2019. Agriculture. In: Annuaire statistique du Cameroun. Institut National de la Statistique, Cameroun, pp. 184–207. Edition 2019. https://ins-cameroun.cm/statistique/annuaire-statistique-du-cameroun-edition-2019/. Accessed 31 January 2024.
Kegah FN, Tchuente HNT, Ndjouenkeu R, Kleih U, Fliedel G, & Forsythe L. 2019. State of knowledge on gari in Cameroon. Food Science & Market. RTBfoods State of Knowledge Report. Ngaoundere. Cameroon. https://doi.org/10.18167/agritrop/00699
Kongsil P, Ceballos H, Siriwan W, Vuttipongchaikij S, Kittipadakul P, Phumichai C, Wannarat W, Kositratana W, Vichukit V, Sarobol E, & Rojanaridpiched C. 2024. Cassava breeding and cultivation challenges in Thailand: Past, present, and future perspectives. Plants. 13(14): 1899. https://doi.org/10.3390/plants13141899
Kuate AF, Ajebesone FN, Suh C, & Boyogueno ADB. 2017. Effects of phytosanitation and cassava (Manihot esculenta) variety on the incidence of cassava mosaic disease and whitefly abundance in a forest zone of Cameroon. Int. J. Agric. Pol. Res. 5(11): 186–191. https://doi.org/10.15739/IJAPR.17.022
Legg JP, Ndjelassili F, & Okao-Okuja G. 2004. First report of cassava mosaic disease and cassava mosaic geminiviruses in Gabon. Plant Pathol. 53: 232. https://doi.org/10.1046/j.1365-3059.2003.00972.x
Legg JP. 2010. Epidemiology of a whitefly-transmitted cassava mosaic geminivirus pandemic in Africa. In: Stansly PA & Naranjo SE (Eds). Bemisia: Bionomics and Management of a Global Pest. pp. 233–257. Springer Dordrecht. Berlin. https://doi.org/10.1007/978-90-481-2460-2
Legg JP, Somado EA, Barker I, Beach L, Ceballos H, Cuellar W, Elkhoury W, Gerling D, Helsen J, Hershey C, Jarvis A, Kulakow P, Kumar L, Lorenzen J, Lynam J, McMahon M, Maruthi G, Miano D, Mtunda K, Natwuruhunga P, Okogbenin E, Pezo P, Terry E, Thiele G, Thresh M, Wadsworth J, Walsh S, Winter S, Tohme J & Fauquet C. 2014. A global alliance declaring war on cassava viruses in Africa. Food Sec. 6: 231–248. https://doi.org/10.1007/s12571-014-0340-x
Likiti O, Diko GR, Mubenga O, & Monde G. 2023. Cassava mosaic disease and abundance of its whitefly vector in North-Western Democratic Republic of Congo. Afri. Crop Sci. J. 31(4): 379–390. https://doi.org/10.4314/acsj.v31i4.1
Macfadyen S, Tay WT, Hulthen AD, Paull C, Kalyebi A, Jacomb F, Parry H, Sseruwagi P, Seguni Z, Omongo AC, Kachigamba D, Otim M, Schellhorn N, Polaszek A, Colvin J, & De Barro P. 2021. Landscape factors and how they influence whitefly pests in cassava fields across East Africa. Landsc. Ecol. 36: 45–67. https://doi.org/10.1007/s10980-020-01099-1
Matic S, da Cunha ATP, Thompson JR, & Tepfer M. 2012. An analysis of viruses associated with cassava mozaic disease in three Angolan Provinces. J. Plant Pathol. 94(2): 443–450. http://www.jstor.org/stable/45156055
McGuire S & Sperling L. 2016. Seed systems smallholder farmers use. Food Sec. 8: 179–195. https://doi.org/10.1007/s12571-015-0528-8
Mouketou A, Koumba AA, Gnacadja C, Zinga-Koumba CR, Meye CA, Ovono APM, Sevidzem SL, Mintsa R, Lepengué AN, & Mavoungou JF. 2022. Cassava mosaic disease incidence and severity and whitefly vector distribution in Gabon. Afri. Crop Sci. J. 30(2): 167–183. https://dx.doi.org/10.4314/acsj.v30i2.5
Namuddu A, Seal S, van Brunschot S, Malka O, Kabaalu R, Morin S, Omongo C, & Colvin J. 2023. Distribution of Bemisia tabaci in different agro-ecological regions in Uganda and the threat of vector-borne pandemics into new cassava growing areas. Front. Sustain. Food Syst. 7: 1068109. https://doi.org/10.3389/fsufs.2023.1068109
Ntawuruhunga P, Okao-Okuja G, Bembe A, Obambi M, Mvila JCA, & Legg JP. 2007. Incidence and severity of cassava mosaic disease in the Republic of Congo. Afri. Crop Sci. J. 15(1): 1–9. https://doi.org/10.4314/acsj.v15i1.54405
Omongo CA, Kawuki R, Bellotti AC, Alicai T, Baguma Y, Maruthi MN, Bua A, & Colvin J. 2012. African cassava whitefly, Bemisia tabaci, resistance in African and South American cassava genotypes. J. Integr. Agric. 11(2): 327–336. https://doi.org/10.1016/S2095-3119(12)60017-3
Permingeat HR, Romagnoli MV, Sesma JI, & Vallejos RH. 1998. A simple method for isolating DNA of high yield and quality from cotton (shape Gossypium hirsutum L.) leaves. Plant Mol. Biol. Rep. 16: 89. https://doi.org/10.1023/A:1007466522028
Sam W, Morris OM, & Richard K. 2021. Whitefly (Bemisia tabaci) preference on selected crops commonly used as intercrops with cassava in Uganda. Academia Letters. Article 1309. https://doi.org/10.20935/AL1309
Sanger F, Nicklen S, & Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74(12): 5463–5467. https://doi.org/10.1073/pnas.74.12.5463
Soro M, Tiendrébéogo F, Pita JS, Traoré ET, Somé K, Tibiri EB, Néya JB, Mutuku JM, Simporé J, & Koné D. 2021. Epidemiological assessment of cassava mosaic disease in Burkina Faso. Plant Pathol. 70(9): 2207–2216. https://doi.org/10.1111/ppa.13459
Sseruwagi P, Sserubombwe WS, Legg JP, Ndunguru J, & Thresh JM. 2004. Methods of surveying the incidence and severity of cassava mosaic disease and whitefly vector populations on cassava in Africa: A review. Virus Res. 100(1): 129–142. https://doi.org/10.1016/j.virusres.2003.12.021
Tchuente HNT, Fouepe GHF, Yao DCM, Mathe S, & Teeken B. 2024. Varietal diversity as a lever for cassava variety development: Exploring varietal complementarities in Cameroon. J. Sci. Food Agric. 104(8): 4808–4817. https://doi.org/10.1002/jsfa.12899
Tize I, Fotso AK, Nukenine EN, Masso C, Ngome FA, Suh C, Lendzemo VW, Nchoutnji I, Manga G, Parkes E, Kulakow P, Kouebou C, Fiaboe KKM, & Hanna R. 2021. New cassava germplasm for food and nutritional security in Central Africa. Sci. Rep. 11: 7394. https://doi.org/10.1038/s41598-021-86958-w
Togni PHB, Marouelli WA, Inoue-Nagata AK, Pires CSS, & Sujii ER. 2018. Integrated cultural practices for whitefly management in organic tamato. J. Appl. Entomol. 142(10): 998–1007. https://doi.org/10.1111/jen.12558
Uzokwe VNE, Mlay DP, Masunga HR, Kanju E, Odeh IOA, & Onyeka J. 2016. Combating viral mosaic disease of cassava in the Lake Zone of Tanzania by intercropping with legumes. Crop Prot. 84: 69–80. https://doi.org/10.1016/j.cropro.2016.02.013
Zinga I, Chiroleu F, Legg J, Lefeuvre P, Komba EK, Semballa S, Yandia SP, Mandakombo NB, Reynaud B, & Lett JM. 2013. Epidemiological assessment of cassava mosaic disease in Central African Republic reveals the importance of mixed viral infection and poor health of plant cuttings. Crop Prot. 44: 6–12. https://doi.org/10.1016/j.cropro.2012.10.010