Endophytic Beauveria bassiana in root, seed, and foliar-treated maize affecting on Spodoptera frugiperda larvae and its parasitization eggs
Article Sidebar
Main Article Content
Abstract
Spodoptera frugiperda is a highly polyphagous pest capable of causing up to 100% yield loss in maize. This study aimed to evaluate the effects of endophytic Beauveria bassiana applied through root drench, seed treatment, and foliar spray on the severity of larval feeding damage and egg parasitization by S. frugiperda. The B. bassiana isolate (JgSPK) used in this study was molecularly identified and deposited in GenBank (acc. no. MZ356494). Results indicated that among the three inoculation methods, seed treatment resulted in the highest endophytic colonization, followed by soil drench and foliar spray. Feeding on leaves colonized by the fungus increased larval mortality and resulted in abnormal pupation. Seed treatment showed the greatest potential in reducing larval feeding severity compared with foliar spray and soil drench applications. However, none of the inoculation methods significantly affected egg parasitization rates. Overall, endophytic B. bassiana reduced larval attack severity but did not influence egg parasitization, indicating compatibility with egg parasitoids. Further research is recommended to evaluate potential effects on larval parasitoids.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Altaf N, Ullah MI, Afzal M, Arshad M, Ali S, Rizwan M, Al-Shuraym LA, Alhelaify SS, & Sayed S. 2023. Endophytic colonization by Beauveria bassiana and Metarhizium anisopliae in maize plants affects the fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae). Microorganisms. 11(4): 1067. https://doi.org/10.3390/microorganisms11041067
Amaro JT, Bueno AdF, Neves PMOJ, Silva DMd, Pomari-Fernandes A, & Favetti BM. 2018. Selectivity of different biological products to the egg parasitoid Telenomus remus (Hymenoptera: Platygastridae). Rev. Bras. Entomol. 62(3): 195–197. https://doi.org/10.1016/j.rbe.2018.04.003
Andrianto E, Fitriana Y, Suharjo R, Swibawa IG, Susilo FX, Semenguk B, & Lestari P. 2024. Re-evaluating the likely presence of Spodoptera frugiperda in Indonesia in 2015 through re-assessment of neglected maize field sample collections from Lampung. Phytoparasitica. 52(4): 70. https://doi.org/10.1007/s12600-024-01190-2
Boaventura D, Martin M, Pozzebon A, Mota-Sanchez D, & Nauen R. 2020. Monitoring of target-site mutations conferring insecticide resistance in Spodoptera frugiperda. Insects. 11(8): 545. https://doi.org/10.3390/insects11080545
Dassou AG, Idohou R, Azandémè-Hounmalon GY, Sabi-Sabi A, Houndété J, Silvie P, & Dansi A. 2021. Fall armyworm, Spodoptera frugiperda (J.E. Smith) in maize cropping systems in Benin: Abundance, damage, predatory ants and potential control. Int. J. Trop. Insect Sci. 41: 2627–2636. https://doi.org/10.1007/s42690-021-00443-5
Faddilah DR, Verawaty M, & Herlinda S. 2022. Growth of fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) fed on young maize colonized with endophytic fungus Beauveria bassiana from South Sumatra, Indonesia. Biodiversitas. 23(12): 6652–6660. https://doi.org/10.13057/biodiv/d231264
Ginting S, Zarkani A, Wibowo RH, & Sipriyadi. 2020. New invasive pest, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) attacking corn in Bengkulu, Indonesia. Serangga. 25(1): 105–117.
Herlinda S, Sinaga ME, Ihsan F, Fawwazi F, Suwandi S, Hasbi, Irsan C, Suparman, Muslim A, Hamidson H, Arsi, Umayah A, & Irmawati. 2021a. Outbreaks of a new invasive pest, the fall armyworm (Spodoptera frugiperda) in South Sumatra, Indonesia. IOP Conf. Ser.: Earth Environ. Sci. 912: 012019. https://doi.org/10.1088/1755-1315/912/1/012019
Herlinda S, Gustianingtyas M, Suwandi S, Suharjo R, Sari JMP, & Lestari RP. 2021b. Endophytic fungi confirmed as entomopathogens of the new invasive pest, the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), infesting maize in South Sumatra, Indonesia. Egypt. J. Biol. Pest Control. 31(1): 124. https://doi.org/10.1186/s41938-021-00470-x
Herlinda S, Gustianingtyas M, Suwandi S, Suharjo R, Sari JMP, Suparman, Hamidson H, & Hasyim H. 2022a. Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn suppressing Spodoptera frugiperda growth. Biodiversitas. 23(11): 6024–6031. https://doi.org/10.13057/biodiv/d231156
Herlinda S, Suharjo R, Sinaga ME, Fawwazi F, & Suwandi S. 2022b. First report of occurrence of corn and rice strains of fall armyworm, Spodoptera frugiperda in South Sumatra, Indonesia and its damage in maize. J. Saudi Soc. Agric. Sci. 21(6): 412–419. https://doi.org/10.1016/j.jssas.2021.11.003
Herlinda S, Suwandi S, Irsan C, Adrian R, Fawwazi F, & Akbar F. 2023. Species diversity and abundance of parasitoids of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) from South Sumatra, Indonesia. Biodiversitas. 24(11): 6184–6190. https://doi.org/10.13057/biodiv/d241140
Donga TK, Vega FE, & Klingen I. 2018. Establishment of the fungal entomopathogen Beauveria bassiana as an endophyte in sugarcane, Saccharum officinarum. Fungal Ecol. 35: 70–77. https://doi.org/10.1016/j.funeco.2018.06.008
Kinyungu SW, Agbessenou A, Subramanian S, Khamis FM, & Akutse KS. 2023. One stone for two birds: Endophytic fungi promote maize seedlings growth and negatively impact the life history parameters of the fall armyworm, Spodoptera frugiperda. Front. Physiol. 14: 1–11. https://doi.org/10.3389/fphys.2023.1253305
Kuate AF, Hanna R, Fotio ARPD, Abang AF, Nanga SN, Ngatat S, Tindo M, Masso C, Ndemah R, Suh C, & Fiaboe KKM. 2019. Correction: Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) in Cameroon: Case study on its distribution, damage, pesticide use, genetic differentiation and host plants. PLoS One. 14(6): e0217653. https://doi.org/10.1371/journal.pone.0217653
Kuzhuppillymyal-Prabhakarankutty L, Ferrara-Rivero FH, Tamez-Guerra P, Gomez-Flores R, Rodríguez-Padilla MC, & Ek-Ramos MJ. 2021. Effect of Beauveria bassiana-seed treatment on Zea mays L. response against Spodoptera frugiperda. Appl. Sci. 11(7): 2887. https://doi.org/10.3390/app11072887
Lestari YA, Verawaty M, & Herlinda S. 2022. Development of Spodoptera frugiperda fed on young maize plant’s fresh leaves inoculated with endophytic fungi from South Sumatra, Indonesia. Biodiversitas. 23(10): 5056–5063. https://doi.org/10.13057/biodiv/d231012
Montezano DG, Specht A, Sosa-Gómez DR, Roque-Specht VR, Sousa-Silva JC, Paula-Moraes SV, Peterson JA, & Hunt TE. 2018. Host plants of Spodoptera frugiperda (Lepidoptera : Noctuidae) in the Americas. Afr. Entomol. 26(2): 286–300. https://doi.org/10.4001/003.026.0286
Mukkun L, Kleden YL, & Simamora AV. 2021. Detection of Spodoptera frugiperda (J . E . Smith) (Lepidoptera: Noctuidae) in maize field in East Flores District, East Nusa Tenggara Province, Indonesia. Intl. J. Trop. Drylands. 5(1): 20–26. https://doi.org/10.13057/tropdrylands/t050104
Prasanna BM, Huesing JE, Eddy R, & Peschke VM. 2018. Monitoring, surveillance, and scouting for fall armyworm. In: Prasanna BM, Huesing JE, Eddy R, & Peschke VM (Eds.). Fall Armyworm in Africa: A Guide for Integrated Pest Management. pp. 11–28. USAID and CIMMYT. Mexico.
Putri QS, Oktapiani W, Herlinda S, & Suwandi S. 2024. Susceptibility of immature Telenomus remus, an egg parasitoid of Spodoptera frugiperda (J.E. Smith), to entomopathogenic fungi from South Sumatra, Indonesia. Egypt. J. Biol. Pest Control. 34(1): 21. https://doi.org/10.1186/s41938-024-00785-5
Russianzi W, Anwar R, & Triwidodo H. 2021. Biostatistics of fall armyworm Spodoptera frugiperda in maize plants in Bogor, West Java, Indonesia. Biodiversitas. 22(6): 3463–3469. https://doi.org/10.13057/biodiv/d220655
Russo ML, Jaber LR, Scorsetti AC, Vianna F, Cabello MN, & Pelizza SA. 2020. Effect of entomopathogenic fungi introduced as corn endophytes on the development, reproduction, and food preference of the invasive fall armyworm Spodoptera frugiperda. J. Pest Sci. 94(3): 859–870. https://doi.org/10.1007/s10340-020-01302-x
Sari JMP, Herlinda S, Suwandi S, & Elfita. 2022. Endophytic fungi from South Sumatra (Indonesia) in seed‑treated corn seedlings affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae). Egypt. J. Biol. Pest Control. 32(1): 103. https://doi.org/10.1186/s41938-022-00605-8
Sartiami D, Dadang, Harahap IS, Kusumah YM, & Anwar R. 2020. First record of fall armyworm (Spodoptera frugiperda) in Indonesia and its occurence in three provinces. IOP Conf. Ser.: Earth Environ. Sci. 468: 012021. https://doi.org/10.1088/1755-1315/468/1/012021
Supartha IW, Susila IW, Sunari AAAAS, Mahaputra IGF, Yudha IKW, & Wiradana PA. 2021. Damage characteristics and distribution patterns of invasive pest, Spodoptera frugiperda (J.E Smith) (Lepidoptera: Noctuidae) on maize crop in Bali, Indonesia. Biodiversitas. 22(6): 3378–3387. https://doi.org/10.13057/biodiv/d220645