DETEKSI KEBERADAAN CITRUS VEIN PHLOEM DEGENERATION (CVPD) DENGAN TEKNIK PCR (POLYMERASE CHAIN REACTION) DI SULAWESI TENGGARA

Abstract

Detection of ocurrence Citrus Vein Phloem Degeneration (CVPD) with PCR (Polymerase Chain Reaction) technique in Southeast Sulawesi.  CVPD disease incidence in Southeast Sulawesi based on symptoms have never been reported.  PCR (polymerase chain reaction) technique  was utilized  to detect CVPD with specific primer forward OI and reverse OI2, specific for amplication of Liberobacter asiaticum DNA.  The result of the reseach indicated that 69% of orange trees in Ladongi, Kolaka District and 65% of orange trees in Lalembuu 1, Konawe Selatan district showed the symptom. PCR technique successfully amplified DNA of bacterium L. asiaticum with  the size of  1100 bp.