The Metarhizium anisopliae Metsch. (Moniliales: Moniliaceae) and Aglaia odorata Lour. (Sapindales: Maliaceae) leaf extract: a potent biopesticide cocktail for cabbage leaf caterpillar management

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Sigit Prastowo
Widi Kusuma Wardhani
Ankardiansyah Pandu Pradana
Ali Wafa
Hardian Susilo Addy
Zul Fauzi Nugroho Hadi

Abstract

Plutella xylostella Linnaeus (Lepidoptera: Plutellidaae) is an important pest on Brassicaceae plants, especially cabbage. The combination of entomopathogenic fungi and botanical pesticides has been widely developed to increase effectiveness effectiveness against P. xylostella . This research aimed to determine the potential of Metarhizium anisopliae and Aglaia odorata Leaf Extract (Ao-LE) as a combined control strategy for P. xylostella. The parameters observed in this research were colony growth, viability of conidia of M. anisopliae, antifeedant activity, mortality, and lethal time 50 (LT50) of P. xylostella . The findings revealed that Ao-LE addition maintained colony growth comparable to that of the control treatment, while significantly enhancing the conidial viability of M. anisopliae. Notably, 0.80% and 1.00% Ao-LE in the M. anisopliae suspension resulted in maximum antifeeding activity. Moreover, incorporating 0.20-1.00% Ao-LE demonstrably improved P. xylostella mortality compared with M. anisopliae alone. The combination of M. anisopliae 107 conidia/mL and 1.00% Ao-LE exhibited the lowest LT50 value of 2.16 days, highlighting the synergistic effect of the combined treatment. In conclusion, this study provides compelling evidence for the compatibility and synergistic potential of M. anisopliae and Ao-LE, presenting a promising strategy for the sustainable management of P. xylostella infestations.

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Prastowo , S.; Wardhani, W. K. .; Pradana, A. P.; Wafa, A. .; Addy, H. S. . . .; Nugroho Hadi, Z. F. The Metarhizium Anisopliae Metsch. (Moniliales: Moniliaceae) and Aglaia Odorata Lour. (Sapindales: Maliaceae) Leaf Extract: A Potent Biopesticide Cocktail for Cabbage Leaf Caterpillar Management. J Trop Plant Pests Dis 2024, 24, 212-222.


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References

Ali S, Farooqi MA, Sajjad A, Ullah MI, Qureshi AK, Siddique B, Waheed W, Sarfraz M, & Asghar A. 2018. Compatibility of entomopathogenic fungi and botanical extracts against the wheat aphid, Sitobion avenae (Fab.) (Hemiptera: Aphididae). Egypt. J. Biol. Pest Control. 28(1): 97. https://doi.org/10.1186/s41938-018-0101-9

Ariani NN, Purwanti E, Rahardjanto A, Fatmawati D, & Purnama FH. 2019. Efektivitas limbah puntung rokok dan ekstrak daun pacar cina (Aglaia odorata Lour.) sebagai insektisida ulat grayak (Spodoptera litura Fabricius.) pada sawi secara in vitro [The effectiveness of cigarette butt waste and Chinese henna leaf extract (Aglaia odorata Lour.) as an insecticide for armyworms (Spodoptera litura Fabricius.) on mustard greens in vitro]. Prosiding Seminar Nasional V 2019: Peran Pendidikan dalam Konservasi dan Pengelolaan Lingkungan Berkelanjutan. pp. 203–210. Please write the editor.

Aw KMS & Hue SM. 2017. Mode of infection of Metarhizium spp. fungus and their potential as biological control agents. J. Fungi. 3(2): 30. https://doi.org/10.3390/jof3020030

Duong NT, Edrada-Ebel R, Ebel R, Lin W, Duong AT, Dang XQ, Nguyen NH, & Proksch P. 2014. New Rocaglamide Derivatives from Vietnamese Aglaia species. Nat. Prod. Commun. 9(6): 833–834. https://doi.org/10.1177/1934578X1400900627

Efri, Aeny TN, Maryono T, & Ronalddi E. 2017. Pengaruh fraksi ekstrak daun pacar cina (Aglaia Odorata L.) terhadap pertumbuhan Colletotrichum capsici penyebab penyakit antraknosa pada cabai (Capsicum annuum L.) secara in vitro [Influence of Aglaia odorata L. leaf extract on in vitro growth of chilli antrachnose fungi (Colletotrichum capsici]. J. Trop. Plants Pests Dist. 17(2): 179–184. https://doi.org/10.23960/j.hptt.217179-184

Fernández-Grandon GM, Harte SJ, Ewany J, Bray D, & Stevenson PC. 2020. Additive effect of botanical insecticide and entomopathogenic fungi on pest mortality and the behavioral response of its natural enemy. Plants. 9(2): 173. https://doi.org/10.3390/plants9020173

Gustiana D, Hidayat C, & Setiati Y. 2019. Pengendalian Plutella xylostella dengan Metarhizium anisopliae dalam mengurangi intensitas kerusakan dan mempertahankan hasil kubis [Control of Plutella xylostella with Metarhizium anisopliae in reducing damage intensity and maintaining cabbage results]. Agroscript. 1(1): 21–28. https://doi.org/10.36423/agroscript.v1i1.184

Hall RG, Bruce I, Cooke NG, Diorazio LJ, Cederbaum F, Dobler MR, & Irving E. 2017. Investigating the structure–activity relationship of the insecticidal natural product Rocaglamide. Chimia (Aarau). 71(12): 845–850. https://doi.org/10.2533/chimia.2017.845

Hidayati NN, Yuliani, & Kuswanti N. 2013. Pengaruh ekstrak daun suren dan daun mahoni terhadap mortalitas dan aktivitas makan ulat daun (Plutella xylostella) pada tanaman kubis [Effect of suren leaf and mahogany leaf extracts on mortality and feeding activity of leaf caterpillars (Plutella xylostella) on cabbage plants]. LenteraBio. 2(1): 95–99.

Hussain A & AlJabr AM. 2020. Potential Synergy between spores of Metarhizium anisopliae and plant secondary metabolite, 1-Chlorooctadecane for effective natural acaricide development. Molecules. 25(8): 1900. https://doi.org/10.3390/molecules25081900

Iqbal M, Khan AR, Rafique M, Suthar V, & Solangi BK. 2015. Comparative efficacy of botanical and synthetic pesticides against major insect pests of cabbage. EAR. III(9): 10038–10057.

Jarlina S, Wibowo L, & Hariri AM. 2015. Kompatibilitas Metarhizium anisopliae dan ekstrak daun babadotan untuk mengendalikan hama kepik hijau di laboratorium [The compatibility of Metarhizium anisopliae and babadotan leaf extract for controlling green ladybug pests in the laboratorium]. J. Agrotek Tropika. 3(2): 266–272. https://doi.org/10.23960/jat.v3i2.2012

Khairunnisa, Martina A, & Titrawani. 2014. Uji efektivitas jamur Metarhizium anisopliae Cps.T.A isolat lokal terhadap hama rayap (Coptotermes curvignathus) [The test of the effectiveness of the local isolate Metarhizium anisopliae Cps.T.A fungus against termites (Coptotermes curvignathus)]. JOM FMIPA. 1(2): 430–438.

Koswanudin D. 2011. Pengaruh ekstrak daun Agalia odorata terhadap perkembangan hama pengisap polong kedelai Nezara viridula dan Riptortus linearis [The effect of Agalia odorata leaf extract on the development of soybean pod sucking pests Nezara viridula and Riptortus linearis]. In: Prayitno BA, Saputra A, Ramli M, Nurmiyati, Prabowo CA, Mumpuni KE, Muzzazinah, & Fatmawati U (Eds.). Prosiding Seminar Nasional VIII Pendidikan Biologi. pp. 153–156. Universitas Sebelas Maret, Surakarta.

Kulkarni S. 2015. Commercialisation of Microbial Biopesticides for the Managementof Pests and Diseases. In: Awasthi LP (Ed.). Recent Advances in the Diagnosis and Management of Plant Diseases. pp. 1–9. Springer India. https://doi.org/10.1007/978-81-322-2571-3

Li TT, Yang J, Huo YY, Zeng ZY, Huang HY, Xu FR, & Dong X. 2022. Control of pathogenic fungi on Panax notoginseng by volatile oils from the food ingredients Allium sativum and Foeniculum vulgare. Lett. Appl. Microbiol. 75(1): 89–102. https://doi.org/10.1111/lam.13706

Maina UM, Galadima IB, Gambo FM, & Zakaria D. 2018. A review on the use of entomopathogenic fungi in the management of insect pests of field crops. JEZS. 6(1): 27–32.

Masyitah I, Sitepu SF, & Safni I. 2017. Potensi jamur entomopatogen untuk mengendalikan ulat grayak Spodoptera litura F. pada tanaman tembakau in vivo [Potential of entomopathogenic fungi to control oriental leafworm Spodoptera litura F. on tobacco plants in vivo]. J. Agroekoteknologi FP USU. 5(3): 484–493.

Nana P, Ekesi S, Nchu F, & Maniania NK. 2016. Compatibility of Metarhizium anisopliae with Calpurnia aurea leaf extracts and virulence against Rhipicephalus pulchellus. J. Appl. Entomol. 140(8): 590–597. https://doi.org/10.1111/jen.12289

Nawaz A, Razzaq F, Razzaq A, Gogi MD, Fernández-Grandon GM, Tayib M, Ayub MA, Sufyan M, Shahid MR, Qayyum MA, Naveed M, Ijaz A, & Arif MJ. 2022. Compatibility and synergistic interactions of fungi, Metarhizium anisopliae, and insecticide combinations against the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae). Sci. Rep. 12(1): 4843. https://doi.org/10.1038/s41598-022-08841-6

Negi P, Rawat BS, & Negi DS. 2016. Antifeedant Activity of extracts & isolated compounds of two Himalayan plants against a polyphagous pest Spodoptera litura. Octa J. Biosci. 4(1): 33–35.

Parajuli S & Paudel S. 2019. Eco-friendly management of diamondback Moth (Plutella xylostella L.) of cabbage (Brassica oleracea var. capitata) in Nepal. IJASBT. 7(3): 304–308. https://doi.org/10.3126/ijasbt.v7i3.25701

Reddy DS & Chowdary NM. 2021. Botanical biopesticide combination concept—a viable option for pest management in organic farming. Egypt. J. Biol. Pest Control. 31(1): 23. https://doi.org/10.1186/s41938-021-00366-w

Ribeiro LP, Blume E, Bogorni PC, Dequech STB, Brand SC, & Junges E. 2012. Compatibility of Beauveria bassiana commercial isolate with botanical insecticides utilized in organic crops in southern Brazil. Biol. Agric. Hortic. 28(4): 223–240. https://doi.org/10.1080/01448765.2012.735088

SNI 8027.2. 2014. Bagian 2: Metarhizium anisopliae [Part 2: Metarhizium anisopliae]. In: Agens Pengendali Hayati (APH). BSN. Jakarta.

Sunarti S. 2017. Pengamatan hama dan penyakit penting tanaman kubis bunga (Brassica oleracea var. botritys L.) dataran rendah [The observation of important pests and diseases of flowering cabbage (Brassica oleracea var. botritys L.) lowland plants]. J. AGROQUA. 13(2): 74–80.

Syamsul E, Lestari D, & SH. 2016. Potensi ekstrak air daun pacar cina (Aglaia odorata) sebagai biolarvasida terhadap larva Aedes Aegypti. [Potential of chinese pacar leaf (Aglaia odorata) water extract as a biolarvicide against Aedes Aegypti larvae]. In: Panggabeasn AS, Tarigan D, & Koesnarpadi S (Eds.). Prosiding Seminar Kimia 2013. pp. 1–9. Samarinda.

Trizelia, Sulyanti E, & Suspalana P. 2018. Virulensi beberapa isolat cendawan entomopatogen Metarhizium spp. terhadap kepik hijau (Nezara viridula) (Hemiptera: Pentatomidae). [The virulence of several isolates of the entomopathogenic fungus Metarhizium spp. against green ladybugs (Nezara viridula) (Hemiptera: Pentatomidae)]. In: Setyawan AD, Sugiyarto, Pitoyo A, Widiastuti A, Windarsih G, & Supatmi (Eds.). Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. pp. 266–269. Biodiversitas. Surakarta.

Trizelia T & Rusli R. 2012. Kompatibilitas cendawan entomopatogen Beauveria bassiana (Bals) Vuill (Deuteromycotina: Hyphomycetes) dengan minyak serai wangi [Compatibility of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) with fragrant lemongrass oil]. J. Trop. Plant Pests Dis. 12(1): 78–84. https://doi.org/10.23960/j.hptt.11278-84

Tunaz H, ER MK, & I?ikber AA. 2019. The Combine Effects of Eicosanoid Biosynthesis Inhibitors and Different Isolates of Metarhizium anisopliae (Metschn.) and Beauveria bassiana (Balsamo) (Deuteromycota: Hyphomycetes) on the Mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) Larvae. KSÜ Tar?m ve Do?a Derg. 22(4): 506–511. https://doi.org/10.18016/ksutarimdoga.vi.525317

Usha H, Babu MN, & Padmaja V. 2014. Detection of compatibility of entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. with pesticides, fungicides and botanicals. IJPAES. 4(2): 613–624.

Wisuda NL, Harjaka T, & Prasetyaningrum ID. 2019. Compatibility between Beauveria bassiana (Bals.) and neem extract against brown plant-hopper (Nilaparvata lugens Stal.). Agric. Sci. 4(1): 9–14. https://doi.org/10.22146/ipas.43074

Zaynab M, Sharif Y, Abbas S, Afzal MZ, Qasim M, Khalofah A, Ansari MJ, Khan KA, Tao L, & Li S. 2021. Saponin toxicity as key player in plant defense against pathogens. Toxicon. 193: 21–27. https://doi.org/10.1016/j.toxicon.2021.01.009