Joint application of B. bassiana and M. anisopliae bioinsecticides for controlling rice bugs and improving rice yields

Main Article Content

Ponijan
Etik Puji Handayani
Nurleni Kurniawati
Rakhmiati
Zulkarnaen

Abstract

The rice bug (Leptocorisa oratorius F.) is a serious pest of head rice (Oryza sativa L.). It feeds on the developing kernels of rice, starting from the heading phase when the panicle is exposed from the boot until the end of the ripening phase, which makes it susceptible to indirect yield loss. Multiple insecticide applications are often made during the hard drought growth stage due to high densities of rice bugs that frequently migrate from nearby harvested fields or as a result of eggs laid by subthreshold populations during the first two weeks of heading. The research was conducted at The Food Crops and Horticulture Laboratory Trimurjo, Central Lampung, from August to October 2020, using a randomized complete block design (RCBD) with 7 bioinsecticide treatments. The objective of the research was to determine the efficacy of integrating biopesticides B. bassiana and M. anisopliae on rice bug mortality and rice yields. The results showed that rice bug mortality increased by 100% with a lethal period of 72 hours after exposure to B. bassiana 5 g L-1 + M. anisopliae 5 g L-1. Additionally, there was a 19.73% increase in the percentage of rice grain maturity (hard dough) and a reduction of 99.4% in damage (empty grain) compared to the control.

Article Details

How to Cite
(1)
Ponijan; Handayani, E. P.; Kurniawati, N. .; Rakhmiati; Zulkarnaen. Joint Application of B. Bassiana and M. Anisopliae Bioinsecticides for Controlling Rice Bugs and Improving Rice Yields. J Trop Plant Pests Dis 2023, 23, 58-64.


Section
Articles

References

Aditya D, Kristiana L, & Awidiyantini R. 2019. Efektifitas aplikasi Beuveria bassiana terhadap hama walang sangit (Leptocorisa acuta L.) pada tanaman padi (Oryza sativa L.) [The Effectiveness of the application of Beauveria bassiana against the rice pest (Leptocorisa acuta L.) on rice (Oryza sativa L.)]. Prosiding SEMNASDAL (Seminar Nasional Sumber Daya Lokal). pp. 331–338. Universiatas Islam Madura. Pamekasan.

Baehaki. 1993. Berbagai Hama Serangga Tanaman Padi [Various Insect Pests of Rice Plants]. Angkasa. Bandung.

Blackman B, Lanka S, Hummel N, Way M, & Stout M. 2015. Comparison of the effects of neonicotinoids and pyrethroids against Oebalus pugnax (Hemiptera: Pentatomidae) in rice. Fla. Entomol. 98(1): 18–26. https://doi.org/10.1653/024.098.0104

Budiyono, Suharto, & Trisyono YA. 2005. Efektivitas Jamur Entomopatogen Beauveria bassiana untuk Pengendalian Hama Walang Sangit [The Effectiveness of the Fungus Entomopathogen Beauveria bassiana for Pest Control of Stink Bugs]. Master Thesis. Universitas Gadjah Mada. Yogyakarta.

Effendy TA, Septiadi R, Salim A, & Mazid A. 2010. Jamur entomopatogen asal tanah lebak di Sumatera Selatan dan potensinya sebagai agensia hayati walang sangit (Leptocorisa oratorius F.) [Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of stink bug (Leptocorisa oratorius F.)]. J. HPT Tropika. 10(2): 154–161. https://doi.org/10.23960/j.hptt.210154-161

Freimoser FM, Screen S, Bagga S, Hu G, & St Leger RJ. 2003. Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts. Microbiology. 149(1): 239–247. https://doi.org/10.1099/mic.0.25761-0

Hasnah, Susanna, & Sably H. 2012. Keefektifan cendawan Beauveria bassiana Vuill terhadap mortalitas kepik hijau Nezara viridula L. pada stadia nimfa dan imago [Effectiveness of fungus Beauveria bassiana on mortality of Nezara viridula on stadia nymph and imago]. J. Floratek. 7(1): 13–24.

Herlinda S, Utama MD, Pujiastuti Y, & Suwandi. 2006. Kerapatan dan viabilitas spora Beauveria bassiana (Bals.) akibat subkultur dan pengayaan media, serta virulensinya terhadap larva Plutella xylostella (Linn.) [Density and viability of spores of Beauveria bassiana (Bals.) Vuill. due to sub-cultures and media enriched, and its virulence against larvae of Plutella xylostella (Linn.)]. J. HPT Tropika. 6(2): 70–78. https://doi.org/10.23960/j.hptt.2670-78

Humairoh D, Hidayat MT, Isnawati, & Prayogo Y. 2013. Pengaruh kombinasi jenis cendawan entomopatogen dengan kerapatan konidia terhadap intensitas serangan larva ulat grayak [The effect of the combination of entomopathogenic fungi and conidia density on the intensity of attack by armyworm larvae]. LenteraBio. 2(1): 19–23.

Hutagalung S, Susilo FX, Indriyati, & Swibawa IG. 2013. Populasi hama dan musuh alami pada pertanaman padi varietas ciherang yang dikelola secara PHT versus konvensional (NonPHT) [Pest populations and natural enemies in Ciherang varieties managed by IPM versus conventional (Non-IPM)]. J. Agrotek Tropika. 1(3): 289–293. https://doi.org/10.23960/jat.v1i3.2052

Koswanudin D & Wahyono TE. 2014. Keefektifan bioinsektisida Beauveria bassiana terhadap hama wereng batang coklat (Nilapavarta lugens), walang sangit (Leptocorisa oratorius), pengisap polong (Nezara viridula) dan (Riptortus linearis) [Effectiveness of Beauveria bassiana bioinsecticide against brown plant hopper (Nilapavarta lugens), stink bug (Leptocorisa oratorius), pod sucker (Nezara viridula) and (Riptortus linearis)]. Seminar Nasional Pertanian Organik. pp. 415–420. Bogor.

Kurniawati N, Meryandini A, & Sunarti TC. 2016. Introduction of actinomycetes starter on coffee fruits fermentation to enhance quality of coffee pulp. Emir. J. Food Agric. 28(3): 188–195. https://doi.org/10.9755/ejfa.2015-05-192

Lacey LA & Goettel MS. 1995. Current developments in microbial control of insect pests and prospects for the early 21st century. Entomophaga. 40: 3–27. https://doi.org/10.1007/BF02372677

Lidova J, Buric M, Kouba A, & Velisek J. 2019. Acute toxicity of two pyrethroid insecticides for five non-indigenous crayfish species in Europe. Vet. Med. 64(3): 125–133. https://doi.org/10.17221/136/2018-VETMED

Manopo R, Salaki CL, Mamajit JEM, & Senewe E. 2013. Padat populasi dan intensitas serangan hama walang sangit (Leptocorisa acuta Thunb.) pada tanaman padi sawah di Kabupaten Minahasa Tenggara [(Population density and paddy bug (Leptocorisa acuta Thunb.) infestation intensity on field paddy plants in the South-East Minahasa Regency]. Cocos. 2(3): 1–13.

Permadi MA. 2012. Patogenisitas Beberapa Isolat Cendawan Entomopatogen Metarhizium spp. terhadap Kumbang Predator Menochilus sexmaculatus F. (Coleoptera: Coccinelidae) [Pathogenicity of Several Isolates of the Entomopathogenic Fungus Metarhizium spp. against the Predatory Beetle Menochilus sexmaculatus F. (Coleoptera: Coccinelidae)]. Master Thesis. Universitas Andalas. Padang.

Prayogo Y, Wedanimbi T, & Marwoto. 2005. Prospek jamur entomopatogen Metarhizium anisopliae untuk mengendalikan ulat grayak Spodoptera litura pada kedelai [Prospects of the entomopathogenic fungus Metarhizium anisopliae to control the armyworm Spodoptera litura on soybeans]. J. Litbang Pertanian. 24(1): 19–26.

Rusly R & Trizelia. 2009. Perbanyakan Beauveria bassiana pada Limbah Organik, Formulasi dan Uji Efektifitas sebagai Bioinsektisida untuk Pengendalian Hama Spodoptera exigua Hubner (Lepidoptera: Noctuidae) [Propagation of Beauveria bassiana on Organic Waste, Formulation and Effectiveness Test as a Bioinsecticide for Pest Control of Spodoptera exigua Hubner (Lepidoptera: Noctuidae)]. Master Thesis. Universitas Andalas. Padang.

Salaki CL & Pelealu J. 2015. Pemanfaatan biopestisida ramah lingkungan terhadap hama Leptocorisa acuta tanaman padi sawah [Utilization of environmentally friendly biopesticides against Leptocorisa acuta pests of lowland rice plants]. Eugenia. 21(3): 127–134. https://doi.org/10.35791/eug.21.3.2015.9702

Sihombing MAEM & Samino S. 2015. Daya repelensi biopestisida terhadap walang sangit (Leptocorisa oratorius F) di laboratorium. Jurnal Biotropika. 3(2): 99–103.

Siwi SS, Yasin A, & Sukarna D. 1981. Slender rice bugs and its ecology and economic threshold. Syiposium on Pest Ecology and Pest Management.

Tabudlong BM, Estroy GFJr, & Juma-ang JR. 2016. Field evaluation of entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillumin for the control of rice bug Leptocorisa oratoriusFabricius. Philippine Journal of Crop Science. 42(1): 114.