KARAKTERISASI RIZOBAKTERI YANG BERPOTENSI MENGENDALIKAN BAKTERI Xanthomonas oryzae pv. oryzae DAN MENINGKATKAN PERTUMBUHAN TANAMAN PADI

Authors

  • Agustiansyah Jurusan Agroteknologi Fakultas Pertanian Universitas LampungJl. Prof. Soemantri Brodjonegoro No.1 Bandar Lampung 35145
  • Satriyas Ilyas Departemen Agronomi dan Hortikultura, Fakultas Pertanian IPB. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680
  • Sudarsono Departemen Agronomi dan Hortikultura, Fakultas Pertanian IPB. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680
  • Muhammad Machmud Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian. Jl. Tentara Pelajar N0.3A, Bogor 16111

DOI:

https://doi.org/10.23960/j.hptt.11342-51

Keywords:

biological control, bacterial leaf bligth, phytostimulator, plant growth regulator

Abstract

Characterization of rhizobacteri having potential to control Xanthomonas oryzae pv.  oryzae and increase plant growth of rice. Rhizobacteria which are isolated from root could produce HCN, siderophore, and plant growth regulator,  induce systemic resistance, and are increase uptake of plant nutrition  such as phosphate.  The objective of this research was to characterize rhizobacteri  as controling agent for  Xanthomonas oryzae pv.oryzae (Xoo) and as plant growth promoter. The results show that the isolates of P. diminuta A6, P. aeruginosa A54, B.subtilis 11 /C, and B. subtilis  5/B  inhibited the growth of Xoo.  B. subtilis 5/B isolate produced the highest siderophore  activity, followed by of P. aeruginosa A54, P. diminuta A6 and B. subtilis 11/C. Only  P. diminuta A6 isolate  produced HCN. The results also showed that all  rhizobacteri  produced IAA i.e.  B.subtilis 5/B (22.10 µg/ml), B. subtilis 11/C (19.05 µg/ml), P. diminuta A6 (8.68 ug/ml), and  P. aeruginosa A54 (2.95 µg/ml). The content of phosphatase enzyme was as folows B.subtilis 5/B (2.78 units/ ml), B.subtilis 11/C (5.7 units/ml), P. diminuta A6 (2.25 units/ml), and P. aeruginosa A54 (5.71 units / ml). Content of peroxidase enzymes in plants that were treated by using isolates was as follows B.subtilis 5/B (1.30 x 10-3 units/mg protein), P. aeruginosa A6 (1.20 x 10-3 units/mg protein), B.subtilis 11/C (1.15 x 10-3 units/mg protein), and P. aeruginosa A54 (1.05 x 10-3 units/mg protein).

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Published

2013-01-18
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