FIRST REPORT OF Begomovirus INFECTION ON PAPAYA IN BENGKULU, INDONESIA

Authors

  • Mimi Sutrawati Study Program of Plant Protection, Faculty of Agriculture, University of Bengkulu
  • Parwito Departement of Agrotechnology, Faculty of Agriculture, Universitas Ratu Samban
  • Priyatiningsih Study Program of Plant Protection, Faculty of Agriculture, University of Bengkulu
  • Agustin Zarkani Study Program of Plant Protection, Faculty of Agriculture, University of Bengkulu
  • Sipriyadi Study Program of Biology, Faculty of Mathematics and Natural Sciences, University of Bengkulu
  • Yenny Sariasih Study Program of Plant Protection, Faculty of Agriculture, University of Bengkulu
  • Dwi Wahyuni Ganefianti Study Program of Agroecotechnology, Faculty of Agriculture, University of Bengkulu

DOI:

https://doi.org/10.23960/j.hptt.12149-55

Keywords:

leaf malformation, polymerase chain reaction, stunting, yellow mosaic

Abstract

A field survey was conducted during 2019, we found a severe systemic yellow mosaic, striped green mosaic on leaves petiole, green spots on the fruit of papaya, leaf malformation, and stunting symptoms on three papaya cultivation area in Rejang Lebong, Kepahiang, Bengkulu Tengah, and Seluma, Bengkulu Province, Indonesia. A begomo-like virus was inferred to be the possible cause of the virus-disease-like symptoms. The study aimed to identify the causal of those typical symptoms on papaya. PCR using universal primer for transcriptional activator protein (TrAp) and replication-associated protein (Rep) gene of Begomovirus successfully amplified the DNA fragments of 900 bp in all 10 detected samples, except for samples with leaf malformation and stunting symptoms. It is indicating that those typical symptoms on papaya is associated with Begomovirus infection, while the causal of leaf malformation and stunting is unknown yet. This work is the first report of Begomovirus infected papaya in Indonesia. Severe disease incidence caused by this pathogen was observed on papaya plants in Bengkulu Province that was in the range of 42–100%. This finding is a precious information to be used for identification, and characterization the species of the virus, determining control strategies against the disease.

References

Chang LS, Lee YS, Su HJ, & Hung TH. 2003. First report of papaya leaf curl virus infecting papaya plants in Taiwan. Plant Dis. 87(2): 204.

Doyle JJ & Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus. 12(1): 13–15.

Fauquet CM & Stanley J. 2003. Geminivirus classification and nomenclature: progress and problems. Ann. Appl. Biol. 142(2): 165–189.

Haerani & Hidayat SH. 2003. Geminivirus penyebab penyakit kuning pada babadotan (Ageratum conyzoides): deteksi molekuler dan studi penularan pada beberapa tanaman Solanaceae. J. Fitopatol. Indones. 7(2): 65–70.

Harmiyati T, Hidayat SH, & Adnan AM. 2015. Deteksi dan respons lima varietas pepaya terhadap tiga isolat Papaya ringspot virus (PRSV). J. AgroBiogen. 11(3): 87–94.

Hidayat SH, Mulyati S, Kadir M, Sulandari S, & Aidawati N. 2002. Tobacco leaf curl virus from Indonesia: its detection and host range study. J. Agrik. 13: 157–161.

Hidayat SH, Chatchawankanpanich O, Rusli E, & Aidawati N. 2006. Begomovirus associated with Pepper yellow leaf curl disease in West Jawa, Indonesia. J. Microbiol. Indones. 11(2): 87–90.

Hidayat SH, Nurulita S, & Wiyono S. 2013. Infeksi Papaya ringspot virus pada tanaman pepaya di Provinsi Nanggroe Aceh Darussalam. J. Fitopatol. Indones. 8(6): 184–187.

Hidayat S, Nurulita S, & Wiyono S. 2012. Temuan penyakit baru infeksi papaya ringspot virus pada tanaman pepaya di Nangroe Aceh Darussalam. J. Fitopatol. Indones. 8(6): 184–187.

Kintasari T, Septariani DWN, Sulandari S, & Hidayat SH. 2013. Tomato yellow leaf curl Kanchanaburi virus penyebab penyakit mosaik kuning pada tanaman terung di Jawa. J. Fitopatol. Indones. 9(4): 127–131.

Li R, Salih S, & Hurtt S. 2004. Detection of geminiviruses in sweetpotato by polymerase chain reaction. Plant Dis. 88(12): 1347–1351.

Listihani, Damayanti TA, Hidayat SH, & Wiyono S. 2018. Karakterisasi molekuler Papaya ringspot virus tipe P pada tanaman mentimun di Jawa. J. Fitopatol. Indones. 14(3): 75–83.

Marie-Jeanne V, Ioos R, Peyre J, Alliot B, & Signoret P. 2000. Differentiation of Poaceae Potyviruses by reverse transcription polymerase chain reaction and restriction analysis. J. Phytopathol. 148(3): 141–151.

Mohammed H, Manglli A, Zicca S, El Hussein A, Mohamed M, & Tomassoli L. 2012. First report of Papaya ringspot virus in pumpkin in Sudan. New Dis. Rep. 26: 26.

Nehra C, Marwal A, Verma RK, Mishra M, Sharma P, & Gaur RK. 2019. Papaya yellow leaf curl virus: A newly identified Begomovirus infecting Carica papaya L. from the Indian Subcontinent. J. Hortic. Sci. Biotechnol. 94(4): 475–480.

Nuhantoro I. 2017. Konstruksi pelacak DNA Papaya ringspot virus berdasarkan gen helper component untuk metode deteksi hibridisasi asam nukleat. Tesis. IPB University. Bogor.

Nurulita S, Hidayat SH, Mutaqin KH, & Thomas J. 2015. Molecular characterization of Begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia. BIOTROPIA. 22(1): 53–60.

Rahim YF, Damayanti TA, & Ghulamahdi M. 2015. Deteksi virus yang menginfeksi kedelai di Jawa. J. Fitopatol. Indones. 11(2): 59–67.

Reddy MK, Venkataravanappa V, Madhuvanthi B, & Jalali S. 2010. Molecular characterization of Begomoviruses associated with papaya leaf curl disease in India. Acta Hortic. 851: 465–472.

Rusli ES, Hidayat SH, Suseno R, & Tjahjono B. 1999. Virus Gemini pada cabai: variasi gejala dan studi cara penularan. Bulletin HPT. 11(1): 26–31.

Saxena S, Hallan V, Singh BP, & Sane PV. 1998. Nucleotide sequence and intergeminiviral homologies of the DNA-A of Papaya leaf curl geminvirus from India. Biochem. Mol. Biol. Int. 45(1): 101–113.

Septariani DN, Hidayat SH, & Nurhayati E. 2014. Identifikasi penyebab penyakit daun keriting kuning pada tanaman mentimun. J. HPT Tropika. 14(1): 80–86.

Shen W, Tuo D, Yang Y, Yan P, Li X, & Zhou P. 2014. First report of Ageratum yellow vein virus associated with a new betasatellite infecting Carica papaya in China. J. Plant Pathol. 96(3): 603–611.

Singh-Pant P, Pant P, Mukherjee SK, & Mazumdar-Leighton S. 2012. Spatial and temporal diversity of begomoviral complexes in papayas with leaf curl disease. Arch. Virol. 157(7): 1217–1232.

Sulandari S. 2004. Karakterisasi biologi, serologi dan analisis sidik jari DNA virus penyebab penyakit daun keriting kuning cabai. Disertasi. Institut Pertanian Bogor. Bogor.

Sutrawati M, Hidayat SH, Suastika G, Sukarno BPW, & Nurmansyah A. 2020. Yellow mosaic disease on soybean. J. Fitopatol. Indones. 16(1): 30–36.

Tennant PF, Fermin GA, & Roye ME. 2007. Viruses infecting papaya (Carica papaya L.): etiology, pathogenesis and molecular biology. Plant Viruses. 1(2): 178–188.

Trisno J, Hidayat SH, Habazar T, Manti I, & Jamsari. 2009. Detection and sequence diversity of Begomovirus associated with yellow leaf curl disease of pepper (Capsicum annuum) in West Sumatera, Indonesia. Microbiology. 3(2): 56–61.

Trisno J, Rifqah RA, & Martinius. 2014. Leaf curl disease of tobacco in West Sumatera. J. Fitopatol. Indones. 10(6): 210–213.

Wiratama IDMP, Wirya GNAS, Adnyani NNP, Nyana IDN, & Suastika G. 2015. Laporan pertama infeksi Begomovirus pada tanaman mentimun di Bali. J. Fitopatol. Indones. 11(5): 175–178.

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Published

2021-02-17
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