MASS PRODUCTION OF ENTOMOPATHOGENIC NEMATODES OF LOCAL ISOLATES AS BIOLOGICAL CONTROL AGENTS  OF COFFEE BERRY BORER (Hypothenemus hampei Ferr.)

Wagiyana; Didik Sulistyanto; Joko Waluyo

doi:10.23960/j.hptt.1198-14

Authors

Wagiyana Department of Pests and Plant Diseases, Faculty of Agriculture, University of Jember
Didik Sulistyanto Department of Pests and Plant Diseases, Faculty of Agriculture, University of Jember
Joko Waluyo Department of Biology, Faculty of Teacher Training and Education, University of Jember

DOI:

https://doi.org/10.23960/j.hptt.1198-14

Keywords:

Mass production, EPNs, control, CBB.

Abstract

Entomopathogenic Nematodes (EpNs) that serve as biological control agents include Steinernema spp and Heterorhabditis spp. EPNs Heterorhabditis indicus (Bromo Isolate) has a high toxicity against larvae Lepidoptera and Coleoptera and was successfully developed for mass production in Biological Control Laboratory of Jember University. These nematodes will be formulated as solid and liquid biopesticides. This research aims: to find EPNs local isolates from smallholder coffee plantations; to identify local and in vivo and in vitro cultured EPNs isolates; to determine the pathogenicity of EpNs local isolate against Coffee Berry Borrer (CBB) Hypothenemus hampei Ferr and to formulate EpNs in vitro culture as a liquid and solid formula. These formula were tested for the patogenicity and viability of EpNs to the larvae of H. hampei, Tenebrio molitor and Galleria melonella. The results showed that the pathogenecity of EpNs isolate to the larvae of CBB in Silo was 30% after 24 hours and 90% after 48 hours of in vivo inoculation. However, the mortality of CBB larvae was only 10% by liquid spraying on the coffee berry. The viability was 524 IJ (Infective Juvenile) on liquid formula packed on polyurethane sponge, and this was higher than that on solid formula (330 IJ).

References

Abdel-Razek AS, Hussein M, & Shehata I. 2018. Isolation and identification of indigenous entomopathogenic nematode (EPN) isolate from egyptian fauna. Arch. Phytopathol. PFL. 51(3–4): 197–206.

Afifah L, Rahardjo BT, & Tarno H. 2013. Eksplorasi nematoda entomopatogen pada lahan tanaman jagung, kedele, dan kubis di Malang serta virulensinya terhadap Spodoptera litura Fabricus. J. HPT. 1(2): 1–9.

Ali F & Wharton DA. 2015. Infective jouveniles of the entomopathogenic nematode, Steinernema feltiae produce cryoprotectants in response to freezing and cold acclimation. J. PLoS ONE. 10(10): e0141810.

Bedding RA. 1984. Large scale production, storage and transport of the insect parasitic nematodes Neoaplectana spp. and Heterorhabditis spp. Ann. Appl. Biol. 104(1): 117–120.

Khairunisa S, Pinem MI, & Zahro F. 2014. Uji efektivitas nematoda entomopatogen sebagai pengendali penggerek pucuk kelapa sawit Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) di laboratorium. J. Online Agroteknol. 2(2): 607–620.

Qodiriyah, Sulistyanto D, & Purwatiningsih. 2015. Agens pengendali hayati nematoda entomopatogen Heterorhabditis sp. dan Steinernema sp. sebagai pengendali hama rayap tanah Captotermes sp. dan Macrotermes sp. di Kabupaten Lumajang. J. I. Dasar. 16(1): 43–48.

Raja KR, Hazir C, Gumus A, Asan C, Karagoz M, & Hazir S. 2015. Efficacy of entomopathogenic nematode Heterorhabditis bacteriophora using different application methods in the presence or absence of a natural enemies. Turk. J. Agric. For. 39(2): 277–285.

Rusniarsyah L, Rauf A, Supramana, & Samsudin. 2015. Patogenisitas dan keefektifan nematoda entomopatogen Heterorhabditis sp. terhadap penggerek umbi kentang Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae). J.Silvikultur Tropika. 6(1): 66–70.

Shapiro-Illan D, Han R, & Dolinksi C. 2012. Entomopathogenic nematode production and application technology. J. Nematol. 44(2): 206–217.

Shapiro-Illan D, Rojas MG, Morales-Ramos JA, & Tedders WL. 2012. Optimization of a host diet for in vivo production of entomopathogenic nematodes. J. Nematol. 44(3): 264–273.

Setyobudi B & Wagiyana. 2008. Pemanfaatan zeolit sebagai bahan pembuatan biopestisida berbahan aktif nematoda Steinernema spp. berbentuk granuler. J. Zeolit Ind. 7(2): 108–112.

Susurluk IA, Kongu Y, & Ulu TC. 2013. Quality control of in vitro produced Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) strains isolated from Turkey. Turk. Entomol. Derg-Tu. 37(3): 283–291.

MASS PRODUCTION OF ENTOMOPATHOGENIC NEMATODES OF LOCAL ISOLATES AS BIOLOGICAL CONTROL AGENTS OF COFFEE BERRY BORER (Hypothenemus hampei Ferr.)

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