ANTIVIRAL ACTIVITY OF CASHEW NUT SHELL EXTRACT AGAINST COWPEA MILD MOTTLE VIRUS ON SOYBEAN

Wuye Ria Andayanie, Wahidin Nuriana, Netty Ermawati

Abstract


Antiviral Activity of Cashew Nut Shell Extract Against Cowpea mild mottle virus on Soybean. Cowpea mild mottle virus (CPMMV) is one of important virus on soybean. Management of CPMMV is usually by controlling its vector using insecticide which has adverse effect on environmental and non target insect. Therefore, it is important to find an alternative control which is environment friendly such as utilizing of plant extract. Cashew nut shell (CNS) is a waste during processing of cashew nut and reported containing some antimicrobe substances.  The research was aimed to evaluated the potency of CNS extract as antiviral against CPMMV. The experiment was designed by Randomized Block Design with 4 replicates and each treatment unit consisted of 10 plants. The application method (prior and post virus inoculation, mixing of virus sap and CNS extract) and concentration of CNS extract (0.75 %; 1.5 %; 3.0 %; 6.0 %) were evaluated the effectiveness of their combination to inhibit CPMMV infection. The application of CNS extract were abled to decreased disease incidence and increase incubation period compared  to untreated with CNS extract. and severity of treated plants significantly. However, plants treated with concentration 3.0 %  and 6.0 % prior virus inoculation and mixing CNS extract with concentration 1.5 %, 3.0 %, and 6.0 % with CPMMV sap showed that the virus titre was negatively detected by ELISA using CPMMV antisera.  It is indicated that the potential of CNS extract as antiviral. All treatments combination able to suppress virus infection without phytotoxicity effect, except CNS 6.0 % treatment.


Keywords


Cowpea mild mottle virus (CPMMV), cashew nut shell extract, antiviral, soybean

References


Aderiye BI, David OM, & Atere VA. 2014. Administration of cashew extracts in the treatment of some infection and diseases. J. Advancement in Medicinal Plant Research. 3(3): 75−86.

Andayanie WR, Sumardiyono YB, Hartono S, & Yudono P. 2011. Incidence of soybean mosaic disease in East Java Province. J. Agrivita. 33(1): 15−21.

Andayanie WR & Adinurani PG. 2014. Seleksi galur dari populasi F4 kedelai yang tahan terhadap penyakit mosaik (Soybean mosaic virus) dan berdaya hasil tinggi. JHPT Tropika. 14(2): 152−159.

Andayanie WR, Santosa V, & Rahayu M. 2017. Resistance to Soybean mosaic virus with high yield on F7 soybean lines. Int. J. of Agric. Biol. 19(2): 226−232.

Andayanie WR, Ermawati N & Iswati R. 2018. Use tillage system and botanical herbicide of cashew nut shell extract on losses nutrient and organic matter in the sloping land. Proceedings of the 4th International Symposium on Applied Chemistry 2018. AIP Conf. Proc. 2024, 020018-1–020018-6. Doi:10.1063/1.5064304.

Andayanie WR & Ermawati N. 2019. Developmental effect of cashew nut shell extract against nymphal instar of silver leaf whitefly (Bemisia tabaci Genn.). IOP C. Ser. Earth Env. 293 (2019) 012039: 1−7.

Andayanie WR, Nuriana W & Ermawati N. 2019. Bioactive compounds and their their antifeedant activity of cashew nut (Anacardium occidentale L.) shell extract against Bemisia tabaci, (Gennadius, 1889) (Hemiptera:Aleyrodidae). J. Acta Agriculturae Slovenica 113(2): 281−288.

Arif M & Hassan S. 2002. Evaluation of resistance in soybean germplasm to soybean mosaic virus Potyvirus under field condition. J. Biol. Sci. 2(9): 601−604.

Cecilio AB, de Faria DB, Oliveira PC, Caldas S, de Oliveira DA, Sobral ME, Duarte MG, Moreira CP, Silva CG, & de Almeida VL. 2012. Screening of Brazilian medicinal plants for antiviral activity against rotavirus. J. Ethiopharmacology. 141(3): 975-981.

Elbeshehy EKF. 2017. Inhibitory activity of different medicinal plants extracts from Thuja orientalis, Nigella sativa L., Zadirachta indica and Bougainvillea spectabilis against Zucchini yellow mosaic virus (ZYMV) infecting citrullus lanatus. J. Biotechnol. Biotec. Eq. 31(2): 270−279.

Garcia NZT, Barbosa GF, Matias R, Pedrinho DR, Bono JAM, & Martini D. 2018. Antifungal potential of cashew nut shell liquid in the control of plant pathogens. Biosci. J. 34(1): 95−103.

Gunaeni N, Wulandari AW & Hudaya A. 2015. Pengaruh bahan ekstrak tanaman terhadap Pathogenesis Related Protein dan asam salisilat dalam menginduksi resistensi tanaman cabai merah terhadap virus keriting. J. Hort. 25 (2): 160−170.

Kristyaningrum VT, Martosudiro M & Hadiastono. 2015. Ekstrak bayam duri (Amaranthus spinosus L.) sebagai penginduksi ketahanan tanaman cabai besar (Capsicum annum L.) terhadap infeksi Cucumber mosaic virus (CMV). JHPT. 3(1): 61−66.

Kurnianingsih L & Damayanti TA. 2012. Lima ekstrak tumbuhan untuk menekan infeksi Bean common mosaic virus pada tanaman kacang panjang. J. Fitopatologi Indonesia 8(6): 155−160.

Madhusudan KN, Vinayarani G, Deepak SA, Niranjana SR, Prakash HS, Singh GP, Sinha AK, & Prasad BC. 2011. Antiviral activity of plant extracts and other inducer against Tobamoviruses infection in bell pepper and tomato plants. Int. J. Plant Pathol. 2(1): 35−42.

Matthews RF. 1992. Fundamental of Plant Virology. Univ. of Auckland New Zealand. Academic Press, Inc, San Diego, California.

Murphy AM, Gilliand A, Wong CE, West J, Singh DP, & Caarr JP. 2001. Signal transduction in resistance to plant viruses. Euro. J. Plant Pathol. 107(1): 121−128.

Petrov NM, Stoyanova M, & Valkova M. 2016. Antiviral activity of plant extract from Tanacetum vulgare against Cucumber mosaic virus and Potato virus Y. J. BioSci. Biotechnol. 5(2): 189−194.

Saleh N. 2007. Sistem produksi kacang-kacangan untuk menghasilkan benih bebas virus. J. Iptek Tanaman Pangan. 2(1): 66−76.

Strange RN. 2003. Introduction to Plant Pathology. New York: John Willey and Sons Ltd.

Sudjaroen Y, Thongkao K & Suwannahong K. 2018. Antioxidant, antibacterial and cytotoxicity activities of cashew (Anacardium occidentale) nut shell waste. Int. J. of Green Pharmacy 12(1): 229−234.

Tavassoli M, Shahraeen N, & Qorbani S. 2008. Detection and some properties of Cowpea mild mottle virus isolated from soybean in Iran. Pakistan J. of Biological Science 11(23): 2624−2628.

Towaha J & Achmadi NR. 2011. Pemanfaatan cashew nut shell liquid sebagai sumber fenol alami pada industri. Buletin RISTRI 2(2): 187−198.




DOI: http://dx.doi.org/10.23960/j.hptt.219%25p

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