THE USE OF GOLDEN SNAIL MEAL TO ENRICH  BACILLUS THURINGIENSIS CULTURE MEDIA AND ITS EFFECT  ON THE BACTERIAL TOXICITY AGAINST SPODOPTERA LITURA

Yulia Pujiastuti; Siti Masyitah; Sarah Dirgahayu; Suparman Surya Hadikusuma; Effendy

doi:10.23960/j.hptt.11823-30

Authors

Yulia Pujiastuti Program Studi Proteksi Tanaman Fakultas Pertanian Universitas Sriwijaya
Siti Masyitah
Sarah Dirgahayu
Suparman Surya Hadikusuma
Effendy

DOI:

https://doi.org/10.23960/j.hptt.11823-30

Keywords:

Bacillus thuringiensis, golden snail meal, Spodoptera litura, storage longevity

Abstract

The use of golden snail meal to enrich Bacillus thuringiensis culture media and its effect on the bacterial toxicity against spodoptera litura. Bacillus thuringiensis is an entomopathogenic bacterium producing spore and protein at sporulation. There has been limited research on using golden snail as protein resource to enrich bacterial culture media. This research was aimed at studying bacterial cell/spore production in culture media made from coconut water media and liquid waste of tofu industry (tofu whey) enriched with golden snail meal (GSM), as well as its toxicity against Spodoptera litura. The research was conducted in the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Sriwijaya University, from June to September 2017. The experiment was arranged in a Factorial Completely Randomized Design (FCRD) with two factors and three replications. The first factor was the addition of GSM (0, 5, 9, 13 and 17 g) and the second was storage longevity (0, 1, and 2 months). Number of test insect (third instar of S. litura) was 30 larvae per treatment. Parameter observed were spore density, larval mortality, and symptom of infected larvae. The results showed that B. thuringiensis cultured in culture media enriched with 13 g golden snail meal produced the highest spore density, amounted to 14.14 x 107spores/ml and caused larval mortality up to 86.67%. After one and two month storage treatments, the spore density in the cultures reduced to 2.51 x 107 and 1.49 x 107 spores/ml, respectively. There was a tendency of reduction in spore density under longer storage of the culture. Survived larvae developed abnormally, failed to transform to pupa or imago.

References

Abdel-Razek AS. 2002. Comparative study on the effect of two Bacillus thuringiensis strains of the same serotype on three coleopteran pests of stored wheat. J. Egypt Soc. Parasitol. 32(2): 415–424.

Amin G, Alotaibi S, Narmen YA, & Saleh WD. 2008. Bioinsecticide production by the bacterium Bacillus thuringiensis. 1. pattern of cell growth, toxin production and by-product synthesis. Arch. Agron. Soil Sci. 54(4): 387–394.

Bagari L, Syedy M, Sharma GP, Sharma P, & Soni P. 2013. Isolation of crystal protein from Bacillus thuringiensis. Int. J. Pure App. Biosci. 1(2): 44–47.

Bernardi D, Bernardi O, Horikoshi RJ, Salmeron E, Okuma DM, & Omoto C. 2016. Biological activity of Bt proteins expressed in different structures of transgenic corn against Spodoptera frugiperda. Ciência Rural 46(6): 1019–1024.

Blanc M, Kaelin P, & Gadani F. 2002. Bacillus thuringiensis (Bt) for the control of insect pests in stored tobacco: a review. Beiträge zur Tabakforschung International-Contributions to Tobacco Research 20(1): 15–22.

Bonab SH, Moravvej G, & Namaghi HS. 2016. Comparative study on the efficacy of Bacillus thuringiensis var. tenebrionis and a neem based insecticide on adults and larvae of Xanthogaleruca luteola (Mull) (Col/: Chyrsomelidae) in laboratory conditions. J. Entomol. Zool. Stud. 4(4): 1122–1125.

Bravo A, Likitvivatanavong S, Gill SS, & Soberón M. 2011. Bacillus thuringiensis: A story of a sucessful bioinsecticide. Insect Biochem. Mol. Biol. 41(7): 423–431.

Chuku LC & Kalagbor GI. 2014. Protein and mineral element content of coconut (Cocos nucifera) water from different species. Am. J. Advanced Drug Delivery 11(2): 5–7.

Devi PSV, Ravinder T, & Jaidev C. 2005. Barley-based medium for the cost-effective production of Bacillus thuringiensis. World J. Microbiol. Biotechnol. 21(2): 173–78.

Dewi FS. 2014. Pemanfaatan tepung keong mas (Pomacea canaliculata) sebagai substitusi tepung ikan pada pakan udang vannamei (Litopenaeus vannamei) terhadap nilai kecernaan serat kasar dan bahan ekstrak tanpa nitrogen (BETN). Skripsi. Universitas Airlangga. Surabaya.

Elleuch J, Zghal RZ, Lacoix MN, Chandre F, Tounsi S, & Jaoua S. 2015. Evidence of two mechanisms involved in Bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: genome dynamic and toxins stability. Microbiol. Res. 176: 48–54.

Fernandes SCM, Freire SCR, Silvestre AJD, Desbrières J, Gandini A, & Neto CP. 2010. Production of coated papers with improved properties by using a water-soluble chitosan derivative. Ind. Eng. Chem. Res. 49(14): 6432–6438.

Galán-Wong LJ, Gamiño-Hernández R, Fernández-Chapa D, García-Díaz G, Myriam A. De La Garza-Ramos, Claudio Guajardo-Barbosa, & Luna-Olvera1 HA. 2017. Persistence of toxic activity of fermentation extracts from Bacillus thuringiensis var. israelensis after more than three decades of storage. Int. J. Microbiol. 2017: 1–6.

Jouzani GS, Abbasalizadeh S, Moradali MF, & Morsali H. 2015. Development of a cost effective bioprocess for production of an Iranian anti-Coleoptera Bacillus thuringiensis strain. J. Agr. Sci. Tech. 17: 1183–1196.

Kurniawati N. 1997. Potensi dan pemanfaatan keong mas sebagai bahan pakan ternak. Balai Besar Penelitian Padi: 47–58.

Marzban R. 2012. Investigation on the suitable isolate and medium for production of Bacillus thuringiensis. J. Biopest. 5(2): 144–147.

Melo ALDA, Soccol VT, & Soccol CR. 2016. Bacillus thuringiensis: mechanism of action, resistance, and new applications: a review. Crit. Rev. Biotechnol. 36(2): 317–326.

Osman GEH, Already R, Assaeedi ASA, Organji SR, El-Ghareeb D, Abulreesh HH, & Althubiani AS. 2015. Bioinsecticide Bacillus thuringiensis a comprehensive review. Egypt J. Biol. Pest Co. 25(1): 271–288.

Paul B, Paul S, & Khan MdA. 2011. A potential economical substrate for large-scale production of Bacillus thuringiensis var. kurstaki for caterpillar control. Biocontrol Sci. Technol. 21(11): 1363–1368.

Prabakaran G, Hoti SL, Manonmani AM, & Balaraman K. 2008. Coconut water as a cheap source for the production of endotoxin of Bacillus thuringiensis var. israelensis, a mosquito control agent. Acta Trop. 105(1): 35–38.

Purnawati R, Sunarti TC, Syamsu K, & Rahayuningsih M. 2014. Characterization of novel Bacillus thuringiensis isolated from Attacus atlas and its growth kinetics in the cultivation media of tofu whey for bioinsecticide production. J. Biol. Agri. Healthcare 4(16): 33–40.

Salazar-Magallon JA, Hernandez-Velazquez VM, Alvear-Garcia A, Arenas-Sosa I, & Pena-Chora G. 2015. Evaluation of industrial by-products for the production of Bacillus thuringiensis strain GP139 and the pathogenicity when applied to Bemisia tabaci nymphs. Bull. Insectology 68(1): 103–1099.

Sarrafzadeh MH. 2012. Nutritional Requirements of Bacillus thuringiensis during different phases of growth, sporulation and germination evaluated by plackett-burman method. Iran J. Chem. Chem. Eng. 31(4): 131–136.

Siregar AZ, Tulus, & Lubis KS. 2017. Utilization of golden snail as alternative liquid organic fertilizer (LOF) on paddy farmers in Dairi, Indonesia. IJSTR. 6(11): 17–21.

Valicente FH, Tuelher EDS, Leite MIS, Freire FL, & Vieira CM. 2010. Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. Revista Brasileira de Milho e Sorgo 9(1): 1–11.

van Frankenhuyzen K, Gringorten JL, Gauthier D, Milne RM, Masson L, & Peferoen M. 1993. Toxicity of activated CryI protein from Bacillus thuringiensis to six forest Lepidoptera and Bombyx mori. J. Invertebr. Pathol. 62(3): 295–301.

THE USE OF GOLDEN SNAIL MEAL TO ENRICH BACILLUS THURINGIENSIS CULTURE MEDIA AND ITS EFFECT ON THE BACTERIAL TOXICITY AGAINST SPODOPTERA LITURA

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