FUNGICIDAL ACTIVITY OF GARLIC (Allium sativum) BULBS EXTRACTS AGAINST PLANTS PHATOGENIC FUNGI

Authors

  • Khairan Syiah Kuala University
  • Aulina Department of Chemistry, Faculty of MIPA, University Syiah Kuala
  • Muhammad Bahi Department of Chemistry, Faculty of MIPA, University Syiah Kuala
  • Cut Nova Eriana Department of Chemistry, Faculty of MIPA, University Syiah Kuala
  • Rina Sriwati Department of Plant Pests, Faculty of Agriculture, Syiah Kuala University

DOI:

https://doi.org/10.23960/j.hptt.11923-32

Keywords:

Allium sativum, Colletotrichum capsici, Fusarium oxysporum f. sp.capsici, phyto fungicidal, plants phatogenic fungi, Sclerotium rolfsi

Abstract

Fungicidal activity of garlic (Allium sativum) bulbs extracts against plants phatogenic fungi. Garlic (Allium sativum Linn) has been known to containing organosulphur compounds. These compounds are convinced to possess antibacterial, antifungal, and anticancer activities. The aim of this study is to determine fungicidal activity of garlic bulb extracts against some plants phatogenic fungi. The paper disc agar difussion technique was used to determine fungicidal activity of garlic bulbs extracts. The results showed that in general garlic bulbs extracts provides fungicidal activities. Calculated EC50 values indicated that ethyl acetate garlic bulbs extract was most active against Colletotrichum capsici, Fusarium oxysporum f. sp.capsici, and Sclerotium rolfsi by EC50 values of 48,6; 50,3; and 51.3% respectively. Meanwhile methanolic garlic bulbs extract was the most active against Sclerotium rolfsi with EC50 values of 24,3%.

Author Biography

Khairan, Syiah Kuala University

Syiah Kuala University, Pharmacy Department

References

REFERENCES

Abdel-Fattah GM, El-Haddad SA, Hafez EE, & Rashad YM. 2011. Induction of defense responses in common bean plants by arbuscular mycorrhizal fungi. Microbiol. Res. 166(4): 268–281.

Abdullah TH, Kandil O, Elkadi A, & Carter J. 1988. Garlic revisited: therapeutic for the major diseases of our times? J. Natl. Med. Assoc. 80(4): 439–445.

Al-Askar AAA. 2012. In vitro antifungal activity of three Saudi plant extracts against some phytopathogenic fungi. J. Plant Protec. Res. 52(4): 458–462.

Alwathnani HA & Perveen K. 2012. Biological control of fusarium wilt of tomato by antagonist fungi and cyanobacteria. Afr. J. Biotechnol. 11(5): 1100–1105.

Amagase H, Petesch BL, Matsuura H, Kusuga S, & Itakura Y. 2001. Intake of garlic and its bioactive components. J. Nutr. 131(3): 955S–962S.

Amagase H. 2006. Clarifying the real bioactive constituents of garlic. J. Nutr. 136(3): 716S–725S.

Anief M. 2010. Ilmu Meracik Obat: Teori dan Praktik. Gadjah Mada University Press, Yogyakarta.

Aniszewki T. 2007. Alkaloid-Secrets of Life. Elsevier. Amsterdam.

Ankri S & Mirelman D. 1999. Antimicrobial properties of allicin from garlic. Microbes Infect. 1(2):125–129.

Baht SV, Nagasampagi BA, & Meenakshi S. 2006. Natural Product: Chemistry and Application. Narosa Publishing House. New Delhi India.

Basuki RS. 1988. Analisis biaya dan pendapatan usaha tani cabai merah di Desa Kemurang Kulon, Brebes. Bul. Penel. Hort. 16(2):115–121.

Bhanderi BB, Yadav MM, & Roy A. 2009. Antifungal drug resistance concerns for veterinarians. Veterinary World. 2(5): 204–207.

Block E. 2010. Garlic and Other Alliums: The Lore and the Science. RSC Publishing. Cambridge. United Kingdom. England.

Boonsang N, Dethoup T, Singburaudom N, Gomes NGM, & Kijjoa A. 2014. In vitro antifungal activity screening of crude extracts of soil fungi against plant pathogenic fungi. Biopest. 7(2): 156-166.

Bosland PW & Kotava EJ. 2012. Peppers: Vegetable and Spice Capsicums. Second ed. CAB International. Wallingford, UK.

Chekki R, Najjaa H, Zouari N, Mathe A, & Bouzouita N. 2016. Detection of organo-sulphur volatiles in Allium sativum by factorial design. Nat. Prod. Chem. Res. 4(2): 1–5.

Cowan MM. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12(4): 564–582.

Departemen Kesehatan Republik Indonesia (Depkes). 1995. Farmakope Indonesia. Edisi Ketiga. Depkes RI. Jakarta.

Direktorat Jenderal Hortikultura. 2013. Statistik Produksi Hortikultura Tahun 2013. Kementerian Pertanian. Jakarta.

Direktorat Jenderal Hortikultura. 2017. Statistik Produksi Hortikultura Tahun 2017. Kementerian Pertanian. Jakarta.

Direktorat Jenderal Pengawas Obat dan Makanan (Dirjen POM). 1997. Materia Medika Indonesia. Jilid 6. Direktorat Jenderal Pengawas Obat dan Makanan. Jakarta.

Divya BJ, Suman B, Venkataswamy M, & Thyagaraju K. 2017. A study on phytochemicals, fungtional groups and mineral composition of Allium sativum (garlic) cloves. Int. J. Curr. Pharm. Res. (9)3: 42–45.

Duriat AS, Gunaeni N, & Wulandari AW. 2007. Penyakit Penting pada Tanaman Cabai dan Pengendaliannya. Balai Penelitian Tanaman Sayuran. Bandung.

Fichtner EJ. 2005. Sclerotium rolfsii Sacc. ‘Kudzu of the Fungal World’. Soilborne Plant Pathogens. Pathogen Profiles. North Carolina State University.

Golkhar MS, Mulik MB, & Laddha KS. 2013. Fate of ß-asarone in Ayurvedic sodhana process of Vacha. J. Ayurveda Integr. Med. Reid. 4(1):19–22.

Harborne JB. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Cetakan Kedua. ITB. Bandung.

Harmatha J. 2000. Chemo-ecological role of spirostanol saponins in the interaction between plant and insects. In: Olezsek W & Marston A (Eds.). Saponins in food, Feedstuffs and Medicinal Plants. pp. 129–141. Proceedings of the Phythochemical Society of Europe. Springer, Dordrecht. The Netherlands.

Haas D & Défago G. 2005. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat. Rev. Microbiol. 3: 307–319.

Hur JS, Ahn SY, Koh YJ, & Lee CI. 2000. Antimicrobial properties of cold-tolerant Eucalyptus species against phytopathogenic fungi and food-borne bacterial pathogens. Plant Path J. 16(5): 286– 289.

Kanan GJ & Al-Najar RA. 2008. In vitro antifungal activities of various plant crude extracts and fractions against citrus post-harvest disease agent Penicillium digitatum. Jordan J. Biol. Sci. 1(3): 89–99.

Kardinan A. 2002. Pestisida Nabati: Ramuan dan Aplikasi. Penebar Swadaya. Jakarta.

Katooli N, Maghsodlo R, & Razavi SE. 2011. Evaluation of eucalyptus essential oil against some plant pathogenic fungi. J. Plant Breed. Crop Sci. 3(2): 41–43.

Khadri S, Boutefnouchet N, & Dekhil M. 2010. Antibacterial activity evaluation of Allium sativum essential oil compared to different Pseudomonas aeruginosa strains in Eastern Algeria. St. Cerc. St. CICBIA. 11(4): 421–428.

Lanzotti V, Barile E, Antignani V, Bonanomi G, & Scala F. 2012. Antifungal saponins from bulbs of garlic, Allium sativum L. var. Voghiera. Phytochemistry. 78: 126–134.

Lawson LD, Wood SG, & Hughes BG. 1991. HPLC analysis of allicin and other thiosulfonates in garlic clove homogenates. Planta Med. 57(3): 263–270.

Macesic N & Wingard JR. 2018. Nystatin. In: Grayson ML (Ed). Kucers’ the Use of Antibiotics: a Clinical Review of Antibacterial, Antifungal, Antiparasitic, and Antiviral Drugs. pp. 144. Seventh Edition. CRC Press. Boca Raton, FL.

Merige B, Mopuri R, Mrali, Krisna T, Meriga B, Mopuri R, & Muralikrisna. 2012. Insecticidal, antimicrobial and antioxidant activities of bulbs extracts of Allium sativum. Asian Pac. J. Trop. Med. 5(5): 391–395.

Morales G, Sierra P, Mancilla A, Parades A, Loyola LA, Gallardo O, & Borquez J. 2003. Secondary metabolites from four medical plants from Nothern Chille: Antimicrobial activity and biotoxicity against Artemia salina. J. Chil. Chem. Soc. 48(2): 13–18.

Morrissey JP & Osbourn AE. 1999. Fungal resistance to plant antibiotics as a mechanism of pathogenesis. Microbiol. Mol. Biol. Rev. 63(3): 708–724.

Nosrati S, Esmaeilzadeh-Hosseini SA, Sarpeleh A, Soflaei-Shahrbabak M, & Soflaei-Shahrbabak Y. 2011. Antifungal activity of spearmint (Mentha spicata L.) essential oil on Fusarium oxysporum f. sp. radicis-cucumerinum the causal agent of stem and crown rot of greenhouse cucumber in Yazd Iran. ICEAE. 15: 52–56.

Nuria MC, Faizatun A, & Sumantri. 2009. Uji aktivitas antibakteri ekstrak etanol daun jarak pagar (Jatropha curcas L.) terhadap bakteri Staphylococcus aureus ATCC 25923, Escherechia coli ATCC 25922 dan Salmonella typhi ATCC 1408. MEDIAGRO. 5(2): 26–37.

Obagwu J & Korsten L. 2003. Control of citrus green and blue moulds with garlic extracts. Euro J. Plant Pathol. 109(3): 221–225.

Pawar VC & Thaker VS. 2007. Evaluation of the anti Fusarium oxysporum f. sp. cicer and anti-Alternaria porri effects of some essential oils. World J. Microbiol. Biotech. 23(8): 1099–1106.

Prapagdee B, Akrapikulchart U, & Mongkolsuk S. 2008. Potential of a soil-borne Streptomyces hygroscopicus for biocontrol of Anthracnose disease caused by 14 Colletotrichum gloeosporioides in orchid. J. Biol. Sci. 8(7): 1187–1192.

Ridawati, Jenie BSL, Djuwita I, & Sjamsuridzal W. 2011. Aktivitas antifungal minyak atsiri jinten putih terhadap Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, dan C. etchelsii MP18. Makara Sains. 15(1): 58–62.

Rustama MM, Rahayuningsih SR, Kusmoro J, & Safitri R. 2005. Uji aktivitas antibakteri dari ekstrak air etanol bawang putih (Allium sativum L.)terhadap bakteri Gram negatif dan bakteri Gram positif. Biotika. 4(2): 1–8.

Sadikin M. 2002. Biokimia Enzim. Wijaya Medika. Jakarta.

Safithri M. 2004. Aktivitas antibakteri bawang putih (Allium sativum) terhadap bakteri Mastitis subklinis secara in vitro dan in vivo pada tikus putih (Rattus novergicus). Tesis. IPB. Bogor.

Shah B & Seth AK. 2010. Textbook of Pharmacognosy and Phytochemistry. Reed Elsevier India Private Lomited, New Delhi.

Saxena M, Saxena J, Nema R, Singh D, & Gupta A. 2013. Phytochemistry of medicinal plants. J Pharmacognosy Phytochemistry. 1(6): 168–82.

Sebaugh JL. 2010. Guidelines for accurate EC50/IC50 estimation. Pharm. Stat. 10(2): 128–134.

Semangun H. 2007. Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Edisi Kedua. Gadjah Mada University Press. Yogyakarta.

Setiyowati H, Surahman M, & Wiyono S. 2007. Pengaruh seed coating dengan fungisida benomil dan tepung curcuma terhadap patogen antraknosa terbawa benih dan viabilitas benih cabai besar (Capsicum annuum L.). Bul. Agron. 35(3): 176–182.

Shafique S, Asif M, & Shafique S. 2015. Management of Fusarium oxysporum f. sp. capsici by leaf extract of Eucalyptus citriodora. Pak. J. Bot. 47(3): 1177–1182.

Sigee DC. 1993. Bacterial Plant Pathology: Cell and Molecular Aspects. Cambridge University Press. United Kingdom.

Sovia E, Sukandar EY, Sigit JI, & Sasongko LDN. 2011. Efek rimpang kunyit (Curcuma longa L.) dan bawang putih (Allium sativum L.) terhadap sensitivitas insulin pada tikus galur Wistar. MKB. 43(4): 153–159.

Taufiq H, Supriyadi, & Sarjiyah. 2015. Pengaruh pemberian ekstrak daun sirih (Piper betle L.) untuk mengendalikan damping-off pada tanaman cabai (Capsicum annum). Planta Tropika J. Agro. Science. 3(1): 60–66.

Turk FM. 2006. Saponins versus plant fungal pathogens. J. Cell Mol. Biol. 5: 13–17.

Untung K. 1996. Pengantar Pengelolaan Hama Terpadu. Universitas Gadjah Mada Press. Yogyakarta.

Wang B , Xu S, Cao Y, Liu F, Zhao X, & Feng. 2018. Fungicidal activity of 10-deacetylbacatin III against Phytophthora capsici via inhibiting lysine biosynthesis. Pestic. Biochem. Phys. 152: 114–121.

World Health Organization (WHO). 2009. Monograf on Medical Plant. Genava. Swiss.

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2019-01-18
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