PENGARUH FRAKSI EKSTRAK DAUN PACAR CINA (AGLAIA ODORATA L.) TERHADAP PERTUMBUHAN COLLETOTRICHUM CAPSICI PENYEBAB PENYAKIT ANTRAKNOSA PADA CABAI (CAPSICUM ANNUUM L.) SECARA IN VITRO

Efri; Titik Nur Aeny; Tri Maryono; Eko Ronalddi

doi:10.23960/j.hptt.217179-184

Authors

Efri Jurusan Proteksi Tanaman, Fakultas Pertanian, Universitas Lampung
Titik Nur Aeny Jurusan Proteksi Tanaman, Fakultas Pertanian, Universitas Lampung
Tri Maryono Jurusan Proteksi Tanaman, Fakultas Pertanian, Universitas Lampung
Eko Ronalddi Jurusan Proteksi Tanaman, Fakultas Pertanian, Universitas Lampung

DOI:

https://doi.org/10.23960/j.hptt.217179-184

Keywords:

Aglaia odorata L., anthracnose, Capsicum annum L., Colletotrichum capsici, growth inhibition

Abstract

Influence of Aglaia odorata L. leaf extract on in vitro growth of chilli antrachnose fungi (Colletotrichum capsici). Pacar cina (Aglaia odorata L.) is known as one of the potential herbs as fungicides. The objective of this study was to determine The effectiveness of various leaf extract fractions of pacar cina as a fungicide to suppress the in vitro growth of C. capsici, The causal agent of anthracnose disease on chili. This study consisted of two sub-experiments. The first experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with ethanol solvent with a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 0.2 % (synthetic fungicides) and control (without fungicide). The Second experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with the solven of n-hexane at a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 2% (synthetic fungicides), and control (without fungicides). The treatmentswerearranged by completely randomized design (CRD) with three replications. Variables measured in this experiment were colony diameter and density of spores of C. capsici. Data were analyzed by analysis of variance (ANOVA) and differences between the mean values was tested by Duncan Multiple Range Test (DMRT) with the level of 5% significant different. Additionally, leaf extract that effectively inhibited the growth of C. capsici was the fraction of the leak extract with 80% ethanol solvent and 10%, 60%, and 90% n-hexanesolvent. The result showed that the leaf extract fraction of pacar cina was potentially used as fungicide to inhibit the growth of C. capsici.

References

Badan Pusat Statistik. 2012. Produksi Cabai Merah Menurut Provinsi. Badan Pusat Statistik Indonesia. Jakarta.

Efri & Prasetyo J. 2011. In vitro effectivity test of active ingredients of biofungicide compound from some extract fractions of mengkudu leaf on growth of Colletotrichum capsici pathogen of antracnose disease on chilli piper. Prosiding Seminar Nasional Sains dan Teknologi IV. pp. 267–278. Bandar Lampung. 29-30 November 2011.

Efri. 2010. Pengaruh ekstrak berbagai bagian tanaman mengkudu (Morinda citrifolia) terhadap perkembangan penyakit antraknosa pada tanaman cabe (Capsicum annuum L.). J. HPT Tropika 10(1): 52–58.

Harnas EDMD, Winarsih S, & Nurdiana. 2009. Efek Antifungi Ekstrak Etanol Rumput Teki (Cyperus rotundus L.) terhadap Candida albicans isolat vaginitis secara in vitro. Laporan Penelitian. Fakultas Kedokteran Universitas Brawijaya. Malang.

Jawetz E, Melnick GE, & Adelberg CA. 2005. Mikrobiologi Kedokteran. Edisi I. Diterjemahkan oleh Penerjemah Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Surabaya.

Pakdeevaraporn P, Wasee S, Taylor PWJ, & Mongkolporn O. 2005. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum. Plant Breeding 124(2): 206–208.

Ridawati, Jenie BSL, Djuwita I, & Sjamsuridzal W. 2011. Aktivitas antifungal minyak atsiri jinten putih terhadap Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, dan C. etchellsii MP18. Makara Sains 15(1): 58–62.

Rubatzky VE. 2000. Sayuran Dunia 3. Institut Teknologi Bandung. Bandung.

Semangun H. 2000. Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Gadjah Mada University Press. Yogyakarta.

Syahnen, Sirait DDN, & Pinem SEB. 2014. Teknik Uji Mutu Agens Pengendali Hayati (APH) di Laboratorium. Laboratorium Lapangan Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan (BBPPTP). Medan.

Soetan KO, Oyekunle MA, Aiyelaagbe O, & Fafunso MA. 2006. Evaluation of the antimicrobial activity of saponins extract of Shorgum bicolor L. Moench. Afr. J. Biotechnol 5(23): 2405–2407.

Sudarmo S. 2005. Teknologi Tepat Guna Pestisida Nabati Pembuatan dan Pemanfaatannya. Kanisius. Yogyakarta.

Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O, & Taylor PWJ. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathol. 57(3): 562–572.

Wang DX & Yang SM. 2013. Chemical constituents from the leaves of Aglaia odorata. Z. Naturforsch. 68(3–4): 82–86.