• Nur Prihatiningsih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Jenderal Soedirman
  • Heru Adi Djatmiko Jurusan Agroteknologi, Fakultas Pertanian, Universitas Jenderal Soedirman
  • Puji Lestari Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jenderal Soedirman




Bacillus subtilis, siderophore, cathecolate, hydroxamate, growth of eggplants, inhibition to pathogens


Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SDCASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively.


Ahmed E & Holmstrom SJM. 2014. Siderophores in environmental research: roles and applications. Microb. Biotechnol. 7: 196–208.

Afreen JM & Chavan MD. 2014. Siderophore Bacillus spp. GN-01 isolated from rhizosphere of ground nut field. Int. J. Pharm. Phytopharmacol. Res. 3(4): 311–313.

Beneduzi A, Ambrosini A, & Passaglia LMP. 2012. Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genet. Mol. Biol. 35(4): 1044–1051.

Choudhary DK & Johri BN. 2009. Interaction of Bacillus spp. and plants-with special reference to induced systemic resistance (ISR). Microb. Res. 164(5): 493–513.

Compant S, Duffy B, Nowak J, Clement C, & Barka EA. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanism of action, and future prospects. Appl. Environ. Microb. 71(9): 4951–4959.

Dawwam GE, Elbeltagy A, Emara HM, Abbas IH, & Hassan MM. 2013. Beneficial effect of plant growth promoting bacteria isolated from the roots of potato plant. Ann. Agric. Sci. 58(2): 195–201.

Egamberdiyeva D & Hoflich G. 2004. Effect of plant growth-promoting bacteria on growth and nutrient uptake of cotton and pea on a semi-arid region of Uzbekistan. J. Arid. Environ.56(2): 293–301.

Ghosh S, Sinha A, & Sahu C. 2007. Isolation of putative probionts from the intestines of Indian mayor carps. Isr. J. Aquacult-Bamid. 59(3): 127–132.

Hu Q-P & Xu J-G. 2011. A simple double-layered chrome azurol S agar (SD-CASA) plate assay to optimize the production of siderophores by a potential biocontrol agent Bacillus. Afr. J. Microbiol. Res. 5(25): 4321–4327.

Kloepper JW, Lifshitz R, & Zablotowicz RM. 1989. Free-living bacterial inocula for enhancing crop productivity. Trends Biotechnol. 7(2): 39–43.

Leelasuphakul W, Hemmanee P, & Chuenchitt S. 2008. Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biol. Technol. 48(1): 113–121.

Lelliot RA & Stead DE. 1987. Methods for The Diagnosis of Bacterial Disease of Plant. British Society for Plant Pathology by Blackwel Scientific Publication, Melbourne.

Lestari P, Prihatningsih N, & Djatmiko HA. 2017. Partial biochemical characterization of crude extract extracellular chitinase enzyme from Bacillus subtilis B298. IOP Conference Series: Materials Science and Engineering. 172 012041.

Morikawa M. 2006. Beneficial biofilm formation by industrial bacteria Bacillus subtilis and related species. J. Biosci. Bioeng. 101(1): 1–8.

Muthukumar A & Venkatesh A. 2013. Exploitaton of fungal and endophytic bacteria for the management of leaf blight of ribbon plant. J. Plant Pathol. Microb. 4(10): 209.

Neilands JB & Nakamura K. 1991. Detection, determination, isolation characterization and regulation of microbial iron chelates. In. Winkelmann G. Handbook of Microbial Iron Chelates. CRC Press. London.

Nudel C, Gonzalez R, Castaneda N, Mahler G, Actis LA. 2001. Influence of Iron on growth, production of siderophore compound, membrane protein, and lipase activity in acinetobacter calcoaceticus BD 413. Microbiol. Res. 155(4): 263–269.

Perez-Miranda S, Cabirol N, George-Tellez R, Zamudio-Rivera LS, & Fernandez FJ. 2007. O-CAS, a fast and universal method for siderophore detection. J. Microbiol. Methods 70 (1): 127–131.

Prihatiningsih N, Arwiyanto T, Hadisutrisno B, & Widada J. 2015. Mekanisme antibiosis Bacillus subtilis B315 untuk pengendalian penyakit layu bakteri kentang. J. HPT Tropika 15(1): 64–71.

Prihatiningsih N & Djatmiko HA. 2016. Enzim amilase sebagai komponen antagonis Bacillus subtilis B315 terhadap Ralstonia solanacearum kentang. J. HPT Tropika 16(1): 10–16.

Radhakrishnan M, Samshath KJ, & Balagurunathan R. 2014. Hydroxamate siderophore from Bacillus sp. SD12 isolated from iron factory soil. Curr. World Environ. 9(3): 990–993.

Saha D, Purkayastha GD, Ghosh A, Isha M, & Saha A. 2012. Isolation and characterization of two new Bacillus subtilis strains from the rhizosphere of eggplant as potential biocontrol agents. J. Plant Pathol. 94 (1): 109–118.

Sharma A, & Johri BN. 2003. Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9, in maize (Zea mays L.) under iron limiting conditions. Microbiol. Res. 158(3): 243–248.

Shin SH, Lim Y, Lee SE, Yang NW, & Rhee JH. 2001. CAS agar diffusion assay for the measurement of siderophore in biological fluids. J. Microbiol. Methods 44(1): 89–95.

Sivasakthi S, Usharani G, & Saranraj P. 2014. Biocontrol potentiality of plant growth promoting bacteria (PGPR)-Pseudomonas fluorescens and Bacillus subtilis: A review. Afr. J. Agric. Res. 9(16): 1265–1277.

Thakaew R & Niamsup H. 2013. Inhibitory activity of Bacillus subtilis BCC 6327 metabolites against growth of aflatoxigenic fungi isolated from bird chili powder. Int. J. Biosci. Biochem. Bioinforma. 3(1): 27–31.



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