KOMPATIBILITAS CENDAWAN METARHIZIUM ANISOPLIAE (METSCHN.) SOROKIN DENGAN NEMATODA ENTOMOPATOGEN STEINERNEMA SP.

Authors

  • Imron Rosyidi Program Studi Agroteknologi, Fakultas Pertanian, Universitas Jember
  • Hari Purnomo
  • Nanang Tri Haryadi
  • Mohammad Hoesain

DOI:

https://doi.org/10.23960/j.hptt.217111-118

Keywords:

compatibility, M. anisopliae, Steinernema sp., Xenorhabdus sp.

Abstract

Compatibility Metarhizium anisopliae (Metschn.) Sorokin with entomopathogenic nematode Steinernema sp. Metarhizium anisopliae (Metschn.) Sorokin and entomopathogenic nematode Steinernema sp. can be used as biological control of insect pests. The objective of this research was to identify compatibility between fungus M. anisopliae with entomopathogenic nematode Steinernema sp. This research was conducted using a completely randomized design (CRD) consisting of 5 treatments and repeated 5 times. The treatment used was M. anisopliae isolates Jombang 1 with Steinernema sp. (M1), M. anisopliae Jombang 2 with Steinernema sp. (M2), M. anisopliae isolates Kediri with Steinernema sp. (M3), M. anisopliae isolates Bondowoso with Steinernema sp. (M4), and M. anisopliae isolates Banyuwangi with Steinernema sp. (M5). Based on this research result, compatibility between the fungus M. anisopliae with entomopathogenic nematode Steinernema sp. was antagonistic. This occurs due to the symbiotic bacteria Xenorhabdus sp. that produced compounds antimycotic and capable to inhibit the growth of fungus M. anisopliae.

References

Alfarizi S. 2014. Pengendalian hayati uret menggunakan nematoda patogen serangga (nps) dan Metarhizium sp. di laboratorium. Skripsi. Universitas Jember. Jember.

Ansari MA, Shah FA, & Butt TM. 2008. Combined use of entomopathogenic nematodes and Metarhizium anisopliae as a new approach for black vine weevil, Otiorhynchus sulcatus, control. Entomol. Exp. Appl. 129(3): 340–347.

Ansari MA, Tirry L, & Moens M. 2005. Antagonism between entomopathogenic fungi and bacterial symbionts of entomopathogenic nematodes. Biocontrol 50(3): 465–475.

Chaerani, Suryadi Y, Priyatno TP, Koswanudin D, Rahmat U, Sujatmo, Yusuf, & Griffin CT. 2007. Isolasi nematoda patogen serangga Steinernema dan Heterorhabditis. J. HPT Tropika 7(1): 1–9.

Dowds B. 1998. Bacterial virulence mechanism. In: Simões N, Boemare N, & Ehlers RU (Eds). Entomopathogenic Nematodes. Pp. 9–19. European Commission, Luxembourg.

Farenhorst M, Knols BGJ, Thomas MB, Howard AFV, Takken W, Rowland M, & N’Guessan R. 2010. Synergy in efficacy of fungal entomopathogens and permethrin against west African insecticide-resistant Anopheles gambiae mosquitoes. Plos One 5(8): 1–10.

Hamilton JT & Attia FL. 1997. Effect of mixture of Bacillus thuringiensis and pesticide on Plutella xylostella and the parasite Thyraella collaris. J. Econ. Entomol. 70(1): 146–148.

Hanudin B, Marwanto, Hersanti, & Muharam A. 2012. Kompatibilitas Bacillus subtilis, Pseudomonas fluorecens, dan Trichoderma harzianum untuk mengendalikan Ralstonia solanacearum pada tanaman kentang. J. Hort. 22(2): 172–179.

Hartati S. 2013. Pengendalian Hama Uret pada Tanaman Tebu. http://www.puslitgula10.com/2013/05/pengendalian-hama-uret-pada-tanaman-tebu_16.html. Diakses tanggal 10 Januari 2017.

Lengyel K, Elke L, Andra´s F, Emilia S, Peter S, & Erko S. 2005. Description of four novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov., and Xenorhabdus szentirmaii sp. nov. Syst. Appl. Microbiol. 28(2): 115–122.

Li J, Chen G, Webster JM, & Czyzewska E. 1995. Antimicrobial metabolites from a bacterial symbiont. J. Nat. Prod. 58(7): 1081–1086.

SAS 1998. Using Statview. SAS Institute Inc., USA.

Shapiro-Ilan DI, Jackson M, Reilly CC, & Hotchkiss MW. 2004. Effects of combining on entomopathogenic fungi or bacterium with entomopathogenic nematodes on mortality of Curculio caryae (Coleoptera: Curculionidae). J. Biol. Control 30(1): 119–126.

Trizelia, Syam U, & Herawaty Y. 2010. Virulensi isolat Metarhizium sp. yang berasal dari beberapa rizosfer tanaman terhadap Crocidolomia pavonana Fabricus (Lepidoptera: Pyralidae). Manggrove 10(2): 51–59.

Webster JM, Chen G, Kaiji-Hu, & Jianxiong-li. 2002. Bacterial metabolites. In: Gaugler R (Ed.). Entomopathogenic Nematology. Pp. 99–114. Cabi Publishing, New York USA.

Xu XM, Jeffries P, Pautasso M, & Jeger MJ. 2011. Combined use of biocontrol agents to manage plant diseases in theory and practice. J. Phytopathology 101(9): 1024–1031.

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2020-09-07
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