POTENSI BAKTERI ENDOFIT DALAM MENEKAN PENYAKIT LAYU STEWART (PANTOEA STEWARTII SUBSP. STEWARTII) PADA TANAMAN JAGUNG

Authors

  • Haliatur Rahma
  • Aprizal Zainal
  • Memen Suharman
  • Meity S Sinaga
  • Giyanto .

DOI:

https://doi.org/10.23960/j.hptt.214121-127

Keywords:

auxin, induced systemic resistance, salicylic acid, siderophore

Abstract

Potential of endophytic bacteria to control stewart wilt disease (Pantoea stewartii subsp. stewartii) in maize. The purpose of this study was to explore endophytic bacteria from seedling, maize roots and grass roots as well as to test the ability of endophytic bacteria which could potentially suppress stewart wilt disease development in maize. Characterization of endophytic bacteria as biocontrol agents including: do not induce HR on tobacco, synthesize IAA, dissolve phosphate, produce siderophores, and antibiotic to Pantoea stewartii subsp. stewartii (Pnss). The results of research shoed 17 isolates of endophytic bacteria potentially as candidate biocontrol agents. Nine isolates were able to produce IAA, siderofores and phosphatase; two isolates produce IAA and phosphatase; six isolates produce IAA. Six isolates ie: AR1, AJ34, AJ15, AJ19, and AJ14 AN6, can increase maize plant resistance and suppress stewart wilt disease severity with a range of 48.95-55.60%.

References

Awais M, Pervez A, Yaqub A, & Shah MM. 2010. Production of antimicrobial metabolites by Bacillus subtilis immobilized in polyacrylamide gel. Pakistan J. Zool. 42(3): 267–275.

Barzanti R, Ozino F, Bazzicalupo M, Gabbrielli R, Galardi F, Gonnelli C, & Mengoni A. 2007. Isolation and characterization of endophytic bacteria from the nickel hyper accumulator plant Alyssum bertolonii. Microb. Ecol. 53(2): 306–316.

Benhamou N, Kloepper JW, Hallmann AQ, & Tuzun S. 1996. Induction defense related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Plant Physiol. 112(3): 919–929.

Chanway CP. 1997. Inoculation of tree roots with plant growth promoting soil bacteria: an emerging technology for reforestation. For Sci. 43(1): 99–112.

Chen C, Bauske EM, Musson G, Rodriguezkaban R, & Kloepper JW. 1995. Biological control of fusarium wilt on cotton by use of endophytic bacteria. Biol Cont. 5:83–91.

Fuente DL, Bajsa N, Bagnasco P, Quagliotto L, Thomashow L, & Arias A. 2004. Antibiotic production by biocontrol Pseudomonas fluorescens isolated from forage legume rhizosphere. http://www.ag.auburn.edu/argentina/pdfmanuscripts/delafuent.pdf . [1 Februari 2012].

Gravel V, Antoun H, & Tweddell RJ. 2007. Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride : possible role of indole acetic acid (IAA). Soil Biol. Biochem. 39(8): 1968–1977.doi: 10.1016/j.soilbio.2007.02.015.

Hallmann J. 2001. Plant interaction with endophytic bacteria. In: Jeger MJ & Spense NJ (Eds.). Biotic Interactions in Plant-Pathogen Association. pp. 87–119. Cab International. Wallingford,UK.

Kuklinsky-Sobral J, WL Araújo, R Mendes IO, Geraldi AA, Pizzirani-Kleiner, & JL. Azevedo. 2004. Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion. Environ. Microbiol. 6 (12):1244–1251.

Liu L, Kloepper JW, & Tuzun S. 1995. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology 85(6): 695–698

Long HH, Schmidt DD, & Baldwin IT. 2008. Native Bacterial Endophytes Promote Host Growth in a Species-Specific Manner; Phytohormone Manipulations Do Not Result in Common Growth Responses, PLoS ONE 3(7): e2702.doi:10.1371/journal.pone.0002702.

Lyon G. 2007. Agents that can elicit induced resistance. In: Walters D, Newton A, & Lyon G (Eds.). Induced Resistance for plant Defence: Sustainable Approach to Crop Protection. pp. 9-29. Blackwell Publishing, Oxford.

Madigan MT, Martinko JM, Parker J. 1997. Biology of Microorganisms. 8th ed. Prentice Hall papper Saddle River Press, London.

Pattern CL & Glick BR. 2002. Role of Pseudomonas putida indoleacetic acid in development of the host plant rootsystem. Appl. Environ. Microbiol. 68: 3795–3801.

Rahma H & Armansyah. 2008. Deteksi Penyakit Stewart oleh Bakteri Pantoea stewartii subsp. stewartii sebagai Penyakit Baru pada Tanaman Jagung (Zea mays): Studi Kasus di Pasaman Barat. J. Manggaro. 9(2): 1–5.

Rahma H. 2013. Penyakit Layu Stewart (Pantoea stewartii subsp. stewartii) pada Jagung dan Upaya Pengendaliannya. [Disertasi]. Bogor. Institut Pertanian Bogor.

Rahma H, Sinaga MS, Surahman M, & Giyanto. 2013. Tingkat Kejadian Penyakit Layu Stewart pada Benih dan Respon beberapa Varietas Jagung terhadap Infeksi Pantoea stewartii subsp. stewartii. J. HPT. Tropika. 13(1): 1–9.

Rao WVBS & Sinha MK. 1962. Phosphate dissolving microorganism in the soil and rhizosphere. Indian J. Sci. 23: 272–278.

Rao NSS. 1994. Mikroorganisme Tanah dan Pertumbuhan Tanaman. Edisi ke dua. Terjemahan Herawati Susilo. UI Press. Jakarta.

Rijavec T, Lapanje A, Dermastia M, & Rupnik M. 2007 Isolation of bacterial endophytes from germinated maize kernels. Can. J. Microbiol. 53(6): 802–808.

Rodríguez H & Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech Adv. 17(4-5): 319–339.

Sessitsch A, Reiter B, & Berg G. 2004. Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities. Can J Microbiol. 50(4): 239–249.

Sigee DC. 1993. Bacterial plant pathology. Cambridge. University Press.

Unterstenhöfer, G. 1963. The basic principles of crop protection field trials. Pflanzenschutz-Nachrichten 16(3): 155–156.

Velusamy P, Immanuel JE, Gnanamanickam SS, & Thomashow L. 2006. Biological control of rice bacterial blight by plant associated bacteria producing 2,4 diacetylphloroglucinol. Can J. Microbiol. 52(1): 56–65.

Whipps JM. 2001. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52(11): 487–511.

Zinniel DK, Lambrecht P, Harris NB, Feng Z, Kuczmarski, Higley P, Ishimaru CA, Arunakumari A, Barletta RG, & Vidaver AK. 2002. Isolation and characterization of endophytic colonizing bacteria from agronomic crop and prairie plants. Appl. Environ. Microbiol. 68(5): 2198–2208.

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Published

2014-06-15
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