TANAMAN INDIKATOR DAN TEKNIK RAPD-PCR UNTUK PENENTUAN BIOTIPE BEMISIA TABACI GENNADIUS (HEMIPTERA: ALEYRODIDAE)

Purnama Hidayat, Noor Aidawati Sri Hendrastuti Hidayat dan Dewi Sartiami

Abstract


Indicator Plant and PCR-RAPD for Biotype Determination of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae).
B. tabaci has been known world wide as a major pest and virus vector of horticulture. In Indonesia the presence of B.
tabaci was reported since 1980 and its role as virus vector in tomato and chilli pepper has becoming more important
recently. Genetic diversity of B. tabaci has been well recognized, but very little information available for diversity of B.
tabaci in Indonesia. This research was conducted in Bogor, West Java from May 2004 to June 2005. The aim of this
research was to initiate basic information regarding genetic diversity of B. tabaci in Indonesia, particularly in Java Island.
Whiteflies population collected from different crops, i.e. tomato, broccoli, chill pepper, eggplant, cucumber, soybean, and
edamame, was evaluated using silverleaf-induction test, and RAPD-PCR. It was evidenced that only B. tabaci population
from broccoli was able to induce silverleaf. Two genetic types of B. tabaci, i.e. biotype B and non B, were identified based
on polymorphism character of DNA. Population from broccoli was belong to biotype B, whereas other populations from
tomato, chill pepper, eggplant, cucumber, soybean, and edamame were belong to biotype non B.

Keywords


Begomovirus, biotipe, RAPD-PCR, Bemisia tabaci

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DOI: http://dx.doi.org/10.23960/j.hptt.181-7

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