PENGARUH INFESTASI VERTICILLIUM LECANII TERHADAP KEPARAHAN PENYAKIT KARAT DAUN KOPI PADA TANAMAN DAN KETERJADIAN KOLONINYA PADA DAUN
Keywords:Verticillium lecanii, Hemileia vastatrix, coffee leaf rust, mycoparasitism
Effect of infestation of Verticillium lecanii on the severity of coffee leaf rust on plants and the incidence of its colony on coffee leaves. V. lecanii often antagonizes H. vastatrix on lesions caused by the pathogen on coffee leaves in the field. The objectives of this study were to evaluate the efficacy of V. lecanii to control disease severity on coffee plants in a green house and to compare the incidence of V. lecanii colonies on coffee leaves naturally infested by V. lecanii in the field and that in the leaves with additional infestation in the laboratory. For efficacy test and additional infestation in the laboratory, conidia were harvested from YMPD medium. Suspension with 107 conidia ml-1 was sprayed into coffee foliage until both surfaces of the leaves were saturated with the suspension. After the leaves were air dried, they were sprayed with urediospores (104 ml-1). The results show that disease severity in plants treated with V. lecanii and control plants was not significantly different (P < 0.05), except that obtained at the fourth week when disease severity on the treated plants was significantly lower that that in control plants. The number of V. lecanii colonies on coffee leaves naturally infested with the biocontrol agent was not increased by additional infestation with V. lecanii conidia in the laboratory.
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