CHARACTERIZATION OF EGGPLANT ENDOPHYTE BACTERIA AND RHIZOBACTERIA AS WELL AS THEIR ANTAGONISTIC ABILITY AGAINST Ralstonia solanacearum

Authors

  • Larasati Puspita Saridewi Faculty of Agriculture, Jenderal Soedirman University
  • Nur Prihatiningsih Faculty of Agriculture, Jenderal Soedirman University
  • Heru Adi Djatmiko Faculty of Agriculture, Jenderal Soedirman University

DOI:

https://doi.org/10.23960/jhptt.220150-156

Keywords:

endophytic bacteria, Ralsonia solanacearum, rhizobacteria

Abstract

Characterization of eggplant endophyte bacteria and rhizobacteria as well as their antagonistic ability against Ralstonia solanacearum. Bacterial wilt caused by Ralsonia solanacearum is one of important diseases causing severe loses in eggplant production. Various strategies were used to manage bacterial wilt, including planting resistant varieties, soil amandement, and soil solarization. However, management of R. solanacearum in eggplant by using endophytic bacteria and rhizobacteria were not been done that much. The objective of this study was to: (1) characterization of endophytic and rhizobacteria; (2) determines the inhibition ability of endophytic and rhizobacteria isolates against R. solanacearum pathogen on eggplant. The laboratory experiment was arranged in completely randomized design with 5 treatments and 5 replications. The double layer method using yeast peptone glucose agar (YPGA) medium was used in vitro test. Based on the morphological characteristics these isolates were suspected as a member of genus Bacillus. Among the isolates used in this study, TK isolate showed the best capability to inhibit growth of R. solanacearum.

References

Achari GA & Ramesh R. 2014. Diversity, biocontrol, and plant growth promoting abilities of xylem residing bacteria from Solanaceous crops. Int. J. Microbiol. 296521: 1–14.

Afizar & Parlina I. 2017. Endophytic bacteria from roots of coffee, and its potential as agent against white root disease Rigidoporus microporus. Bioleuser. 1(2): 54–62.

Breed RS, Murray EDG, & Smith NR. 1957. Bergey’s Manual of Determinative Bacteriology. Seventh Edition. The Williams & Wilkins Company. Baltimore.

Davis WW & Stout TR. 1971. Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Appl. Microbiol. 22(4): 659–665.

Dewi MK, Ratnasari E, & Trimulyono G. 2014. Aktivitas antibakteri ekstrak daun majapahit (Crescentia cujete) terhadap pertumbuhan bakteri Ralstonia solanacearum penyebab penyakit layu. LenteraBio. 3(1): 51–57.

Djatmiko HA, Arwiyanto T, Hadisutrisno B, & Sunarminto BH. 2007. Potensi tiga genus bakteri dari tiga rizosfer tanaman sebagai agensia pengendali hayati penyakit lincat. JIPI. 9(1): 40–47.

OEPP/EPPO. 2004. EPPO Standards PM 7/21(1) Diagnostic protocol-Ralstonia solanacearum. Bulletin OEPP/EPPO Bulletin. 34: 173–178.

Genin S & Boucher C. 2002. Ralstonia solanacearum: secrets of a major pathogen unveiled by analysis of its genome. Mol. Plant Pathol. 3(3): 111–118.

Ghosh S, Sinha A, & Sahu C. 2007. Isolation of putative probionts from the intestines of Indian major carps. Isr. J. Aquacult.-Bamid. 59(3): 127–132.

Hatmanti A. 2000. Pengenalan Bacillus spp. Oseana. 25(1): 31–41.

Iqlima D, Ardiningsih P, & Wibowo MA. 2017. Aktivitas antibakteri isolat bakteri endofit B2D dari batang tanaman yakon (Smallanthus sonchifolius (Poepp. & Endl.) H. Rob.) terhadap bakteri Staphylococcus aureus dan Salmonella thypimurium. JKK. 7(1): 36–43.

Kusumawati DE, Pasaribu FH, & Bintang M. 2014. Aktivitas antibakteri isolat endofit dari tanaman miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli. Curr. Biochem. 1(1): 45–50.

Kuswinanti T, Baharuddin, & Sukmawati S. 2014. Efektivitas isolat bakteri dari rizosfer dan bahan organik terhadap Ralstonia solanacearum dan Fusarium oxysporum pada tanaman kentang. J. Fitopatol. Indones. 10(2): 68–72.

Moorman GW. 2011. Bacterial wilt-Ralstonia solanacearum. PennState Extention. https://extension.psu.edu/bacterial-wilt-ralstonia-solanacearum. Accessed on July 2020.

Mukamto, Ulfah S, Mahalina W, Syauqi A, Istiqfaroh L, & Trimulyono G. 2015. Isolasi dan karakterisasi Bacillus sp. pelarut fosfat dari rhizosfer tanaman Leguminosae. Sains & Mat. 3(2): 62–68.

Nafiah H, Pujiyanto S, & Raharjo B. 2017. Isolasi dan uji aktivitas kitinase isolat bakteri dari kawasan geotermal Dieng. Bioma. 19(1): 22–29.

Nasrun, Christanti, Arwiyanto T, & Mariska I. 2007. Karakteristik fisologis Ralstonia solanacearum penyebab penyakit layu bakteri nilam. J. Litri. 13(2). 43–48.

Nawangsih AA, Widjayani T, & Anisa Y. 2014. Kelimpahan bakteri rizosfer pada sistem PHT-bio intensif serta kemampuan antagonismnya terhadap Sclerotium rolfsii pada kedelai. J. HPT Tropika. 14(2): 110–120.

Pranoto E, Fauzi G, & Hingdri. 2014. Isolasi dan karakterisasi bakteri endofit pada tanaman teh (Camellia sinensis (L.) O. Kuntze) produktif dan belum menghasilkan klon GMB 7 dataran tinggi. Biospecies. 7(1): 1–7.

Pratiwi RH. 2017. Mekanisme pertahanan bakteri patogen terhadap antibiotik. J. Pro-Life. 4(3): 418–429.

Prihatiningsih N & Djatmiko HA. 2016. Enzim amilase sebagai komponen antagonis Bacillus subtilis B315 terhadap Ralstonia solanacearum Kentang. J. HPT Tropika. 16(1): 10–16.

Prihatiningsih N, Djatmiko HA, & Lestari P. 2017. Aktivitas siderofor Bacillus subtilis sebagai pemacu pertumbuhan dan pengendali patogen tanaman terung. J. HPT Tropika. 17(2): 170–178.

Rahmawanto DG, Muhibuddin A, & Aini LQ. 2015. Pengaruh faktor abiotik kimia tanah terhadap supressifitas tanah dalam mengendalikan penyakit layu bakteri (Ralstonia solanacearum) pada tanaman tomat (Lycopersicon esculentum Mill). J. HPT. 3(2): 1–8.

Ramesh R & Phadke GS. 2012. Rhizosphere and endophytic bacteria for the suppression of eggplant wilt caused by Ralstonia solanacearum. Crop Prot. 37: 35–41.

Ramesh R, Ghanekar A, & Joshi M. 2009. Pseudomonads: major antagonistic endophytes to suppress bacterial wilt pathogen, Ralstonia solanacearum in eggplant (Solanum melanogena L.). World J. Microb. Biot. 25(1): 47–55.

Reinhold-Hurek B & Hurek T. 2011. Living inside plants: bacterial endophytes. Curr. Opin. Plant Biol. 14(4): 435–443.

Setyari AR, Aini LQ, & Abadi AL. 2013. Pengaruh pemberian pupuk cair terhadap penyakit layu bakteri (Ralstonia solanacearum) pada tanaman tomat (Lycopersicum esculentum Mill.). J. HPT. 1(2): 80–87.

Soesanto L. 2013. Pengantar Pengendalian Hayati Penyakit Tanaman. Edisi Kedua. Rajawali Pers, Jakarta.

Sukmawaty E, Masri M, Putri SU, & Nurzakiyah. 2016. Aktivitas antibakteri ekstrak dan bakteri endofit makro alga Caulerpa racemosa L. asal perairan Puntondo terhadap Staphylococcus aereus dan methicilin resistant Staphylococcus aereus (MRSA). Prosiding Seminar Nasional from Basic Science to Comprehensive Education. pp. 174–179. Universitas Islam Negeri Alauddin, Makassar.

Downloads

Published

2020-09-16
Read Counter : 11 times
PDF Download : 2 times