TEST OF BLOOD DISEASE BACTERIUM (BDB) TRANSMISSION BY POTENTIAL INSECT VECTORS

Article Sidebar

PDF
Published: Jun 16, 2020
DOI: https://doi.org/10.23960/j.hptt.12071-77
Keywords:
Drosophilidae heliconia transmission test vector

Main Article Content

Betty Sahetapy
Faculty of Agriculture, Pattimura University, Indonesia
Nina Maryana
Faculty of Agriculture, IPB University, Indonesia
Syafrida Manuwoto
Faculty of Agriculture, IPB University, Indonesia
Kikin H. Mutaqin
Faculty of Agriculture, IPB University, Indonesia
Fransina Latumahina
Faculty of Agriculture, Pattimura University, Indonesia

Abstract

Blood disease bacterium (BDB) is one of the important diseases in banana and a major obstacle in developing and increasing banana production in Indonesia. The purpose of this study was to prove the ability of the Drosophilidae insect as a vector in transmitting BDB. The research was conducted at the Insect Biosystematics Laboratory and Plant Bacteriology Laboratory, Department of Plant Protection, Faculty of Agriculture, IPB University. Drosophilidae insects were taken from the field and then reared in laboratory by being fed with ripe bananas to obtain offspring that are free from diseases or pathogens. Imago of the Drosophilidae from rearing was fed by inoculum sources which was infected banana, then inoculated into healthy plants. The plants used were healthy and flowering, heliconia. The results showed that the Drosophilidae insects were able to transmit BDB to heliconia plants that showed symptoms, brownish flower colors and falling flower crowns. Detection of BDB isolated from flower parts and the inside parts of the insects used in transmission test using the PCR method showed positive results.

Article Details

How to Cite
(1)
Sahetapy, B.; Maryana, N.; Manuwoto, S.; H. Mutaqin, K.; Latumahina, F. TEST OF BLOOD DISEASE BACTERIUM (BDB) TRANSMISSION BY POTENTIAL INSECT VECTORS. J Trop Plant Pests Dis 2020, 20, 71-77.
Issue
Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

Antonacci R, Tritto P, Cappucci U, Fanti L, Piacentini L, & Berloco M. 2017. Drosophilidae monitoring in Apulia (Italy) reveals Drosophila suzukii as one of the four most abundant species. Bull. Insectology. 70(1): 139–146.

Dietzgen RG, Mann KS, & Johnson KN. 2016. Plant virus-insect vector interactions: current and potential future research directions. Viruses. 8(303): 1–21.

Hadiwiyono. 2011. Blood bacterial wilt disease of banana: the distribution of pathogen in infected plant, symptoms, and potentiality of diseased tissues as source of infective inoculums. Nusantara Biosci. 3(3): 112–117.

Latumahina F, Borovanska M, Musyafa, Sumardi, Putra NS, & Janda M. 2015. Ants of Ambon Island-diversity survey and checklist. ZooKeys. 472: 43–57.

Mairawita, Habazar T, Hasyim A, Nazir N, & Suswati. 2012. Potensi serangga pengunjung bunga sebagai vektor penyakit darah bakteri (Ralstonia solanacearum Phylotipe IV) pada pisang di Sumatera Barat. JEI. 9(1): 38–47.

Montong VB & Salaki CL. 2019. Serangga pengunjung bunga pisang kepok di Kabupaten Minahasa Selatan sebagai pembawa Ralstonia solanacearum Filotipe IV (penyebab penyakit darah pisang). Jurnal ENFiT. 1(1): 17–28.

Opina N, Tavner F, Hollway G, Wang JF, Li TH, Maghirang R, Fegan M, Hayward AC, Krishnapillai V, Hong WF, Holloway BW, & Timmis JN. 1997. A novel method for development of species and strain-specific DNA probes and PCR primers for identifying Burkholderia solanacearum (formerly Pseudomonas solanacearum). Asia Pac. J. Mol. Biol. Biotechnol. 5(1): 19–30.

Sagar V, Gurjar MS, Arjunan J, Bakade RR, Chakrabarti SK, Arora RK, & Sharma S. 2014. Phylotype analysis of Ralstonia solanacearum strains causing potato bacterial wilt in Karnataka in India. Afr. J. Microbiol. Res. 8(12): 1277–1281.

Sahetapy B, Uluputty MR, & Naibu L. 2019. Identifikasi lalat buah (Bactrocera spp.) asal tanaman cabai (Capsicum annuum L.) dan belimbing (Averrhoa carambola L.) di Kecamatan Salahutu Kabupaten Maluku Tengah. Jurnal Agrikultura. 30(2): 63–74.

Stulberg MJ, Shao J, & Huang Q. 2015. A multiplex PCR assay to detect and differentiate select agent strains of Ralstonia solanacearum. Plant Dis. 99: 333–341.

Wijaya IN. 2007. Penularan penyakit CVPD (Citrus Vein Phloem Degeneration) oleh Diaphorina citri Kuwayama (Homoptera: Psyllidae) pada tanaman jeruk siam. Agritrop. 26(4): 140–146.

Wijaya IN, Adiartayasa W, Sritamin M, & Yuliadhi KA. 2010. Dinamika populasi Diaphorina citri Kuwayama (Homoptera: Psyllidae) dan deteksi CVPD dengan teknik PCR. JEI. 7(2): 78–87.