UTILIZATION OF BETEL LEAVES EXTRACT TO PREVENT THE GROWTH OF Fusarium oxysporum f.sp. capsici CAUSING FUSARIUM WILT DISEASE IN BELL PEPPER

Authors

  • Ni Wayan Anik Leana Agrotechnology Study Program, Faculty of Agriculture, Universitas Jenderal Soedirman
  • Dewa Ngurah Suprapta Agrotechnology Study Program, Faculty of Agriculture, Universitas Udayana

DOI:

https://doi.org/10.23960/j.hptt.12126-33

Keywords:

betel, antifungal, F. oxysporum f.sp. capsici, natural pesticide

Abstract

Betel (Piper betle Linn.) is commonly used in Indonesian traditional medicine due to its antimicrobial properties, thus it is often used as an ingredient in natural pesticides. The effectiveness of betel leaf extract as a control for Fusarium oxysporum in several plants showed it’s potential to control the fungi in bell pepper. F. oxysporum f.sp. capsici is one of the important pathogens causing Fusarium wilt disease in bell pepper. It is one of the most devastating plant diseases due to its ability to cause a crop failure. The test on the inhibitory capacity of betel leaf extract on the growth of F. oxysporum f.sp. capsici were carried out by growing the fungi on PDA mixed with various concentrations of betel leaf extracts. The results showed that betel leaf extract treatment at 0.02% concentration was able to inhibit the growth of F. oxysporum f.sp. capsici in PDA at 95.54%. The full inhibition of colony growth (100%) was achieved in the extract treated with the concentration of 0.03%. Meanwhile treatment of 0.17% betel leaf extract was able to inhibit the growth of F. oxysporum f.sp. capsici on bell pepper stems. Following this result, fractionation of the betel leaf extract by column chromatography was conducted, resulting in 44 fractions. The bioassays of those fractions showed that, there were seven fractions that reveal inhibition capability against F. oxysporum f.sp. capsici.

References

Abdelmajeed NA, Danial EN, & Ayad HS. 2013. The effect of environmental stress on qualitative and quantitative essential oil of aromatic and medicinal plants. Arch. Sci. 66(4): 100–120.

Ali I, Khan FG, Suri KA, Gupta BD, Satti NK, Dutt P, Afrin F, Qazi GN, & Khan IA. 2010. In vitro antifungal activity of hydroxychavicol isolated from Piper betle L. Ann. Clin. Microbiol. Antimicrob. 9: 1–9.

Andrews JM. 2001. Determination of minimum inhibitory concentrations. J. antimicrob. chemother. 48. Suppl. SI: 5–16.

Aoki Y, Trung NV, & Suzuki S. 2019. Impact of Piper betle leaf extract on grape downy mildew: effects of combining 4-allylpyrocatechol with eugenol, á-pinene or â-pinene. Plant Protect. Sci. 55(1): 23–30.

Arsih DW, Panggeso J, & Lakani I. 2015. Uji ekstrak daun sirih dan cendawan Trichoderma sp. dalam menghambat perkembangan Fusarium oxysporum f.sp lycopersici penyebab penyakit layu Fusarium pada tanaman tomat. Natural Science: Jurnal Science and Technology. 4(3): 355–368.

Cahyono B. 2003. Cabai Paprika Teknik Budi Daya dan Analisis Usaha Tani. Kanisius, Yogyakarta.

Darmadi AAK. 2015. Isolasi dan Identifikasi Senyawa Aktif Ekstrak Daun Kayu Manis (Cinnamomum burmanni Blume) dan Uji Efektivitasnya dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat yang disebabkan oleh Jamur Fusarium oxysporum Forma Specialis lycopersici. Laporan Akhir Penelitian Disertasi. Universitas Udayana, Bali.

Datta A, Ghoshdastidar S, & Singh M. 2011. Antimicrobial property of Piper betel leaf against clinical isolates of bacteria. IJPSR. 2(3): 104–109.

Foo LW, Salleh E, & Mamat SNH. 2015. Extraction and qualitative analysis of Piper betle leaves for antimicrobial activities. IJETSR. 2: 1–8.

Gnanasekaran P, Salique SM, Panneerselvam A, & Umamagheswari K. 2015. In vitro biological control of Fusarium oxysporum f. sp. cubense by using some Indian medicinal plants. Int. J. Curr. Res. Aca. Rev. 3(11): 107–116.

Morales G, Sierra P, Mancilla A, Paredes A, Loyola LA, Gallardo O, & Borquez J. 2003. Secondary metabolites from four medicinal plants from Northern Chile: antimicrobial activity and biotoxicity against Artemia salina. J. Chil. Chem. Soc. 49(1): 44–49.

Murugesan S, Vijayakumar R, & Panneerselvam A. 2011. Antifungal activity of medicinal plants against plant pathogenic fungus Fusarium oxysporum. J. Pharm. Res. 4(3): 843–844.

Neela FA, Sonia IA, & Shamsi S. 2014. Antifungal activity of selected medicinal plant extract on Fusarium oxysporum Schlechtthe causal agent of Fusarium wilt disease in tomato. Am. J. Plant Sci. 5(18): 2665–2671.

Patil RS, Harale PM, Shivangekar KV, Kumbhar PP, & Desai RR. 2015. Phytochemical potential and in vitro antimicrobial activity of Piper betle Linn. leaf extracts. J. Chem. Pharm. Res. 7(5): 1095–1101.

Pagare S, Bhatia M, Tripathi N, Pagare S, & Bansal YK. 2015. Secondary metabolites of plants and their role: overview. Curr. Trends Biotechnol. Pharm. 9(3): 293–304.

Phabiola TA. 2004. Penggunaan Ekstrak Beberapa Jenis Tumbuhan untuk Mengendalikan Penyakit Layu Pisang pada Pembibitan dari Bonggol. Tesis. Universitas Udayana, Bali.

Rahardjo M, Rosita SMD, Fathan R, & Sudiarto. 1999. Pengaruh cekaman air terhadap mutu simplisia pegagan (Centella asiatica L.). J. Littri. 5(3): 92–97.

Rongai D, Pulcini P, Pesce B, & Milano F. 2014. Antifungal activity of some botanical extracts on Fusarium oxysporum. Open Life Sci. 10(1): 409–416.

Sanit S. 2016. Antifungal activity of crude extracts of some medicinal plants against Fusarium sp., the pathogen of dirty panicle disease in rice. J. Med. Plants Res. 10(19): 248–255.

Singha IM, Kakoty Y, Unni BG, Kalita MC, Das J, Naglot A, Wann SB, & Singh L. 2011. Control of Fusarium wilt of tomato caused by Fusarium oxysporum f.sp. lycopersici using leaf extract of Piper betle L.: a preliminary study. World J. Microbiol. Biotechnol. 27(11): 2583.

Singburaudom N. 2015. Hydroxychavicol from Piper betel leave is an antifungal activity against plant pathogenic fungi. JBiopest. 8(2): 82–92.

Subrata IM & Rai IGA. 2019. Aktivitas fungisida ekstrak daun sirih (Piper betle L.) kultivar beleng terhadap jamur Fusarium oxysporum f.sp. vanillae penyebab penyakit busuk batang pada vanili. J. EMASAINS. 8(1): 41–50.

Suprapta DN & Khalimi K. 2012. Anti-fungal activities of selected tropical plants from Bali Island. Phytopharmacology. 2(2) 265–270.

Trisilawati O & Pitono J. 2012. Pengaruh cekaman defisit air terhadap pembentukan bahan aktif pada purwoceng. Bul. Littro. 23(1): 34–47.

Downloads

Published

2020-12-25
Read Counter : 41 times
PDF Download : 27 times