DETECTION OF GENES RESISTANT TO BACTERIAL LEAF BLIGHT IN RICE CULTIVARS FROM SITUBONDO AND JEMBER, INDONESIA

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Rasmiyana .,
Hardian Susilo Addy
Erlia Narulita

Abstract

Detection of genes resistant to bacterial leaf blight in rice cultivars from Situbondo and Jember, Indonesia. Xanthomonas oryzae pv. oryzae (Xoo), causing bacterial leaf blight, is a destructive pathogen that significantly affects rice production. The use of resistant varieties is the most effective and economical strategy to reduce the impact of the disease. This study aimed to analyze the genetic basis of resistance to Xoo in rice. The incidence and severity of bacterial leaf blight were assessed in the field through a diagonal random sampling method. PCR was used to detect resistant genes in rice with eight Xa-specific primers. The presence of Xa genes and environmental conditions were statistically analyzed to determine whether the disease incidence and disease severity were related to average temperature and rain intensity. The means for disease incidence and severity at the generative stage were higher than those at the vegetative stage. The 12 rice cultivars were categorized into four groups based on the presence of Xa gene sequences; most cultivars had Xa10 and Xa13, and one cultivar had five Xa genes. Disease incidence and severity were weakly correlated to the presence of resistance genes, as well as to environmental factors. Most rice cultivars from the Regencies of Jember and Situbondo carry Xa10 and Xa13 resistance genes.

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(1)
., R.; Addy, H. S.; Narulita, E. DETECTION OF GENES RESISTANT TO BACTERIAL LEAF BLIGHT IN RICE CULTIVARS FROM SITUBONDO AND JEMBER, INDONESIA. J Trop Plant Pests Dis 2019, 19, 127-134.


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References

Agrios G. 2005. Plant Pathology, 5th Edition. Elsevier Academic Press, Massachusetts.

Aktar W, Sengupta D & Chowdhury A. 2009. Impact of pesticides use in agriculture: Their benefits and hazards. Interdisc.Toxicol., 2 (1): 1–12.

Almeida DM, Almadanum MC, Lourenço T, Abreu IA, Saibo NJ & Oliveira MM. 2016. Screening for abiotic stress tolerance in rice: salt, cold, and drought. Methods Mol. Biol, 1398 : 155–182.

Antony G, Zhou J, Huang S, Li T, Liu B, White F & Yang B. 2010. Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3. Plant Cell, 22 (11): 3864–3876.

Arunakumari K, Durgarani C V., Satturu V, Sarikonda KR, Chittoor PDR, Vutukuri B, Laha GS, Nelli APK, Gattu S, Jamal M, Prasadbabu A, Hajira S & Sundaram RM. 2016. Marker-assisted pyramiding of genes conferring resistance against bacterial blight and blast diseases into Indian rice variety MTU1010. Rice Sci., 23 (6): 306−316.

Cohen SP, Liu H, Argueso CT, Pereira A, Cruz CV, Verdier V & Leach JE. 2017. RNA-Seq analysis reveals insight into enhanced rice Xa7-mediated bacterial blight resistance at high temperature. PLoS ONE, 12 (11): e0187625.

Ilao S & Lastimosa P. 1985. Research Techniques in Ccrops, Book Series No. 35. Philippine Council for Agriculture, Forestry and Natural Resources Research and Development, National Science and Technology Authority, Los Baños, Laguna, Philippines.

Jeung JU, Heu SG, Shin MS, Vera Cruz CM & Jena KK. 2006. Dynamics of Xanthomonas oryzae pv. oryzae populations in Korea and their relationship to known bacterial blight resistance genes. Phytopathology, 96 (8): 867–875.

Khaeruni A, Taufik M, Wijayanto T & Johan E. 2014. Development of bacterial leaf blight disease inoculated on three varieties of paddy rice at various growth stage. J. Fitopatol. Indones., 10 (4): 119–125.

Mew T & Misra J. 1994. A Manual of Rice Seed Health Testing. International Rice Research Institute, Los Baños, Philippines.

Naqvi SAH. 2019. Bacterial leaf blight of rice: An overview of epidemiology and management with special reference to Indian sub-continent. Pak. J. Agri. Res., 32 (2): 359–380.

Niño-Liu DO, Ronald PC & Bogdanove AJ. 2006. Xanthomonas oryzae pathovars: Model pathogens of a model crop. Mol. Plant Pathol., 7 (5): 303–324.

Nisha S, Revathi K, Chandrasekaran R, Kirubakaran SA, Sathish-Narayanan S, Stout MJ & Senthil-Nathan S. 2012. Effect of plant compounds on induced activities of defense-related enzymes and pathogenesis-related protein in bacterial blight disease susceptible rice plant. Physiol. Mol. Plant Pathol., 80 : 1–9.

Perumalsamy S, Bharani M, Sudha M, Nagarajan P, Arul L, Saraswathi R, Balasubramanian P & Ramalingam J. 2010. Functional marker-assisted selection for bacterial leaf blight resistance genes in rice (Oryza sativa L.). Plant Breed., 129 (4): 400–406.

Sambrook J & Russell D. 2001. Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, New York.

Singh AK, Dharmraj E, Nayak R, Singh PK & Singh NK. 2015. Identification of bacterial leaf blight resistance genes in wild rice of eastern India. Turk. J. Bot., 39 (6): 1060–1066.

Suparyono, Sudir Dan S. 2004. Pathotype profile of Xanthomonas oryzae pv. oryzae, isolates from the rice ecosystem in Java. Indones. J. Agric. Sci., 5 (2): 63–69.

Suryadi Y, Samudra IM, Priyatno TP, Susilowati DN, Lestari P, Fatimah F & Kadir TS. 2016. Determination of pathotypes from Indonesian Xanthomonas oryzae pv. oryzae population causing bacterial leaf blight and their reactions on differential rice. Makara J. Sci., 20 (3): 109–118.

Susanto U & Sudir. 2012. Genotypic resistance of rice to Xanthomonas oryzae pv. oryzae pathotype III, IV, VIII. Jurnal Penelitian Pertanian Tanaman Pangan, 31 (2): 108–116.

Vikal Y & Bhatia D. 2017. Genetics and Genomics of Bacterial Blight Resistance in Rice. IntechOpen. Website: https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics.

Wahyudi AT, Meliah S & Nawangsih A. 2011. Xanthomonas oryzae pv. oryzae causing bacterial leaf blight in rice: isolation, characterization, and study of mutagenesis with transposons. Makara J. Sci., 15 (1): 89–96.

Wang J, Tian D, Gu K, Yang X, Wang L, Zeng X & Yin Z. 2017. Induction of Xa10-like genes in rice cultivar nipponbare confers disease resistance to rice bacterial blight. Mol. Plant-Microbe Interact., 30 (6): 466–477.

Webb KM, Garcia E, Cruz CMV & Leach JE. 2010. Influence of rice development on the function of bacterial blight resistance genes. Eur. J. Plant Pathol., 128 (3): 399–407.

Yoshimura S, Yamanouchi U, Katayose Y, Toki S, Wang ZX, Kono I, Kurata N, Yano M, Iwata N & Sasaki T. 1998. Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation. PNAS, 95 (4): 1663–1668.