coffee, coffee berry borer, biological control, Beauveria basiana, Metarhizium anisopliae


Laboratory Evaluation of Local Isolates of Beauveria bassiana and Metarhizium anisopliae against Coffee Berry Borer, Hyphotenemus hampei, using spryaing method. Beauveria bassiana Bals (Vuillemin) and Metarhizium anisopliae (Metschnikoff) Sorokin are two species of fungi that have been studied widely and used as biological control of various insect pests, including coffee berry borer (CBB), Hyphotenemus hampei Ferrari. Laboratory studies were done in June – July 2017, to evaluate local isolates of both fungi species against CBB, the most destructive insect pest of coffee worldwide, including in Indonesia. B. bassiana was isolated from dead sweet potato weevil (Cylas formicarius) and green stink bug (Nezara viridula), and from soil. M. anisopliae was isolated from soil. The pure cultures of the fungi were mass-cultured on corn based media. The conidia harvested from 4 week colonies was hand sprayed directly upon CBB adults (females) and damaged coffee berry at the concentration of 109 ml-1. In additions, the conidia of B. bassiana isolated from C. formicarius alone was diluted to 106- 109 ml-1 and applied to damaged coffee berry. The results showed that when the conidia were applied directly upon the insects, the mortality of beetle were higher in B. bassiana than in M. anisopliae treatments. Pooled data indicated that B. bassiana isolated from death insects significantly caused higher mortality than did M. anisopliae isolated from soil. Beetle mortality was 76.7 and 80.0% for B. basiana, and 60.3 and 60% for M. anysopliae treatments. Application upon damage coffee berry indicated much lower mortality. Application of higher concentration upon damaged coffee berry resulted higher mortality, but data are not consistent. The mortality of CBB larvae was much less and negligible even at the highest conidia concentration.


Alba-Alejandre I, Alba-Tercedor J, & Vega FE. 2018. Observing the devastating coffee berry borer (Hypothenemus hampei) inside the coffee berry using microcomputed tomography. Scientific Reports. 8(17033).

Aristizabal LF, Lara O, & Arthurs1 SP. 2012. Implementing an Integrated Pest Management Program for Coffee Berry Borer in a Specialty Coffee Plantation in Colombia. J. Integ. Pest Mngmt. 3(1): G1–G5.

Baker PS, Jackson JAF, & Murphy ST. 2002. Natural enemies, natural allies. In: Ospina HF (Ed.). Project completion report of the integrated management of coffee berry borer project, CFC/ICO/02 (1998–2002). The commodities press. CABI commodities.

Balakrishnan MM & Naik PR. 2014. Infectivity of ten Metarhizium anisopliae isolates to the coffee berry borer Hypothenemus hampei (Coleoptera: Curculionidae). J. Entomol. Zool. Studies. 2(5): 246–249.

Belay YC & Tenkegna TA. 2017. Bioassay and pilot mass production of entomopathogenic fungus, Beauveria bassiana for the control of coffee berry borer (Hypothenemus hampei: Scolytidae), Ferrari. J. Appl. Biosci. 117: 11669–11683.

Benavides P, Góngora C, & Bustillo A. 2012. IPM Program to control coffee berry borer Hypothenemus hampei, with emphasis on highly pathogenic mixed strains of Beauveria bassiana, to overcome insecticide resistance in Colombia. In: Perveen FK (Ed.). Insecticide - Advances in Intergrated Pest Management. pp. 511–540. Intech Open Access Publisher.

Brun LO & Suckling DM. 1992. Field selection for endosulfan resistance in coffee berry borer (Coleoptera: Scolytidae). J. Econ. Entomol. 85(2): 325–334.

Brun LO, Marcillaud C, Gaudichon V, & Suckling DM. 1989. Endosulfan resistance in Hypothenemus hampei (Coleoptera: Scolytidae) in New Caledonia. J. Econ. Entomol. 82(5): 1311–1316.

Brun LO, Suckling DM, Roush RT, Gaudichon V, Pheisler H, & Robertson JL. 1995. Genetics of Endosulfan Resistance in Hypothenemus hampei (Coleoptera: Scolytidae): Implications for Mode of Sex Determination. J. Econ. Entomol. 88(3): 470–474.

Brun LO, Stuart J, Gaudichon V, Aronstein K, & Ffrench-Constant RH. 1995. Functional haplodiploidy: a mechanism for the spread of insecticide resistance in an important international insect pest. Proc. Natl. Acad. Sci. USA. 92(21): 9861–9865.

Celestino FN, Pratissoli D, Machado LC, Junior HGJDS, De-Queiroz VT, & Mardgan L. 2016. Control of coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) with botanical insecticidesand mineral oils. Acta Sci. Agron. 38 (1): 1–8.

Damon A. 2000. A review of the biology and control of the coffee berry borer, Hypothenemus hampei (Coleoptera: Scolytidae). Bull. Entomol. Research 90(6): 453–465.

Dembilio O, Quesada-Moraga E, Santiago-Alvarez C, & Jacas JA. 2010. Potential of an indigenous strain of the entomopathogenic fungus Beauveria bassiana as a biological control agent against the Red Palm Weevil, Rhynchophorus ferrugineus. J. Invertebr Pathol. 104 (3): 214–221.

Dufour BP & Fre´rot B. 2008. Optimization of coffee berry borer, Hypothenemus hampei Ferrari (Col., Scolytidae), mass trapping with an attractant mixture. J. Appl. Entomol. 132: 591–600.

Feng, MG, Poprawski TJ, & Khachatourians GG. 1994. Production, Formulation and Application of the Entomopathogenic Fungus Beauveria bassiana for Insect Control: Current Status. Biocontrol Sci. Tech. 4(1): 3–34.

Infante F. 2018. Pest Management strategies against the coffee berry borer (Coleoptera: Curculionidae:colytinae). J. Agr. Food Chem. 66(21): 5275–5280.

Jaramillo J, Borgemeister C, & Setamou M. 2006. Field superparasitism by Phymastichus coffea, parasitoid of theadult coffee berry borer Hypothenemus hampei. Entomol. Exp. Appl. 119(3): 231–237.

Jaramillo J, Chabi-Olaye A, Poehling HM, Kamonjo C, & Borgemeister C. 2009. Development of an improved laboratory production technique for the coffee berry borer Hypothenemus hampei, using fresh coffee berries. Entomol. Exp.Appl. 130: 275–281.

Mascarin GM, Stefan M, & Jaronski T. 2016. The production and uses of Beauveria bassiana as a microbial insecticide. World J. Microbiol. Biotechnol. 32:177.

Patocka J. 2016. Bioactive metabolites of entomopathogenic Fungi Beauveria bassiana. Mil. Med. Sci. Lett. 85(2): 80–88.

Posada F & Vega FE. 2005. A new method to evaluate the biocontrol potential of single spore isolatesof fungal entomopathogens. J. Insect Sci. 5: 37.

Pérez J, Infante F, Poinar G, Castillo A, & Vega FE. 2015. Natural parasitism of Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae) on the coffee berry borer in Chiapas, Mexico. Biocontrol Sci. Tech. 25(5): 608–612.

Perez-Lachaud G, Hardy ICW, & Lachaud JP. 2002. Insect gladiators: competitive interactions between three species of bethylid wasps attacking the coffee berry borer, Hypothenemus hampei (Coleoptera: Scolytidae). Biological Control 25(3): 231–238.

Rosa WDL, Alatorre R, Barrera J F& Toriello C. 2000. Effect of Beauveria bassiana and Metarhizium anisopliae (Deuteromycetes) upon the Coffee Berry Borer (Coleoptera: Scolytidae) Under Field Conditions. J. Econ. Entomol. 93(5): 1409–1414.

Rosa WDL, Alatorre R, Trujillo J, & Barrera JF. 1997. Virulence of Beauveria bassiana (Deuteromycetes) strains against the coffee berry borer (Coleoptera: Scolytidae). J. Econ. Entomol. 90(6): 1534–1538.

Sitanggang S, Sitepu SF, & Lubis L. 2017. Survei serangan hama penggerek buah kopi (Hypothenemus hampei Ferr.) berdasarkan faktor kultur Tteknis di Kabupaten Tapanuli Utara . J. Agroekoteknologi FP USU 5 (4): 816–823.

Sparks TC & Nauen R. 2014. IRAC: mode of action classification and insecticide resistance management. Pestic. Biochem. Physiol. 121:122–128.

Vega FE. 2004. Coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). In: Capinera JL (Ed.). Encyclopedia of Entomology. pp. 575–576. Kluwer Academic Publishers.

Vega FE, Infante F, Johnson AJ. 2015. The genus Hypothenemus, with emphasis on H. hampei, the coffee berry borer. In: Vega FE & Hofstetter W (Eds.). Bark Beetles: Biology and Ecology of Native and Invasive Species. pp. 427–494. Academic Press.

Wiryadiputra S, Cilas C, & Morin JP. 2008. The effectiveness of brocap trap to control coffee berry borer (Hypothenemus hampei (Ferr.) in Indonesia. Proceedings of the 22nd International Conference of Coffee Science. pp.1405–1408. Campinas-SP, Brazil.

Wiryadiputra S. 2012. Effectiveness cyantraniliprole against coffee berry borer (Hypothenemus hampei) on arabica coffee. Pelita Perkebunan 28(2): 100–110.



Read Counter : 396 times
PDF Download : 42 times